抗胆固醇抗性等离子体通过表面多糖体生物合成双重增强细菌毒性和抗生素耐药性
Eunbyeol Ahn1,2,3, Jinshil Kim1,3,4,5, Junyao Jiang6
1Department of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Nature communications
|November 12, 2025
概括
调动的胆固醇抗性基因 (mcr-1) 质粒通过调节表面多糖的产生来增强大肠杆菌的致病性和胆固醇抗性. 这凸显了耐药性基因如何意外地增加细菌毒性.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 胆固醇抗药基因 (mcr-1) 质粒在多药耐药格拉姆阴性细菌中的患病率很高.
- mcr-1等离子体对细菌生理学和毒性的影响尚未完全理解.
研究的目的:
- 为了研究mcr-1等离子体如何影响细菌生理学和毒性.
- 阐明mcr-1等离子体增强大肠杆菌病原性的机制.
主要方法:
- 在大肠杆菌中进行的等离子体获取实验.
- 对基因调节 (wec operon) 和表面多糖生物合成的分析.
- 病毒性评估的小鼠模型.
- 对mcr等离子体的基因组分析.
主要成果:
- 获得mcr-1等离子体增加了大肠杆菌的抗微生物耐药性和致病性.
- 转录调节器EcaR和MCR-1合作激活wec操作子,驱动肠细菌常见抗原 (ECA) 和O链多糖化合物合成.
- 这些多糖增强了胆汁的抗性和毒性,并进一步增加了胆素的抗性.
- 基因组分析显示,mcr-1和ecaR在常见的等离子体类型 (IncI2,IncX4) 上同时发生.
结论:
- mcr-1等离子体可以通过协调耐药性和毒性基因表达来重塑细菌表面.
- 横向基因转移mcr-1等离子体可以导致细菌致病性增加.
- 这些发现揭示了通过塑介导的表面多糖的调节来连接抗微生物耐药性和毒性的一种机制.
更多相关视频
相关概念视频
01:30Development of Antibiotic Resistance
1.2K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.2K
01:28Plasmids
1.1K
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
1.1K
01:28Gene Regulation in Microbial Communities: Quorum Sensing
486
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
486
00:57Antibiotic Selection
59.3K
Overview
59.3K
01:31Defense Against Bacterial Pathogens
2.6K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.6K
00:46Genomic DNA in Prokaryotes
48.2K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
48.2K