相关实验视频

Updated: Jan 6, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

预测IG:T细胞表皮质免疫性的一种可解释的预测因子

Roc Farriol-Duran^1,2, Christian Domínguez-Dalmases³, Albert Cañellas-Solé³

¹Barcelona Supercomputing Center, Barcelona, Spain. roc.farriol@bsc.es.

Genome medicine

|November 12, 2025

概括

通过整合抗原处理和物理化学性质,PredIG增强了用于免疫疗法的T细胞表位发现. 这种可解释的预测因子提高了对病原体和癌症新抗原的免疫性查成功率.

科学领域:

免疫学和生物信息学
计算生物学和药物发现

背景情况:

细胞毒性T细胞对于对抗病原体和瘤至关重要.
鉴定免疫性T细胞表位是基于抗原的免疫疗法至关重要的.
目前用于表位发现的方法在吞吐量和准确性方面面临挑战.

研究的目的:

开发一种可解释的T细胞表皮质免疫性预测剂.
提高T细胞表位体免疫性查的成功率.
为了确定T细胞表皮质免疫性超出HLA-I结合亲和力的关键驱动因素.

主要方法:

在17448个-HLA-I等位基因对上训练有素的PredIG,据报道具有免疫性.
集成在的特性包括抗原处理,HLA-I结合和物理化学特性.
利用XGBoost模型和SHAP进行预测和可解释性分析.

主要成果:

预测IG实现了致病原体和非正规癌症抗原的尖端免疫性查成功率 (ISSR).
分析显示,抗原和物理化学性质之间具有平衡的特征重要性.
优先考虑超越顶部结合候选人的额外免疫新抗原.

结论:

预测IG得分完善和扩大T细胞表位的优先级,用于感染和癌症免疫疗法.
PredIG提供了前所未有的免疫学解释性,突出了超出HLA-I结合的驱动因素.
通过开源工具和Web服务器,PredIG促进了高通量抗原的发现.

更多相关视频

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

相关概念视频

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...

Cross-reactivity

Cross-reactivity