相关实验视频
Updated: Jul 15, 2026

07:08
Detection of Histone Modifications in Plant Leaves
Published on: September 23, 2011
25.3K
结构,进化和表达模式分析揭示了bHLH成员与树中粉耐药性相关
Xiaokang Fan1,2, Xiaoling Tang1,2, Yiying Lu1,2
1College of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China.
Plants (Basel, Switzerland)
|November 13, 2025
概括
研究人员在树中确定了204个基本螺旋环螺旋 (bHLH) 基因,发现11个对粉状菌有反应. 三个关键基因被确定为它们在疾病抵抗中的作用,为繁殖提供了目标.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 基本的螺旋环-螺旋环 (bHLH) 转录因子对于植物生长和耐压力至关重要.
- 粉 (Erysiphe quercicola) 对树 (Hevea brasiliensis) 的种植构成重大威胁.
- 了解树中的bHLH基因对于开发抗病品种至关重要.
研究的目的:
- 在树 (Hevea brasiliensis) 品种CATAS73397.7.中识别和描述bHLH转录因子家族.
- 为了研究HbbHLH基因在对粉状菌感染的反应中的作用.
- 为了确定潜在的候选基因来增强树的抗病能力.
主要方法:
- 全基因组识别和分类HbbHLH基因.
- 分析RNA测序数据以确定对感染有反应的基因.
- 遗传学分析,促进体分析和定量实时PCR (qRT-PCR) 验证.
主要成果:
- 鉴定出了204种HbbHLH转录因子,并将其分为15个子家族,其扩张是由全基因组重复驱动的.
- 11 HbbHLH基因在粉状真菌感染时显示出差异性表达.
- HbbHLH87和HbbHLH162-2是下调的,而HbbHLH25是上调的;这些基因对甲基莉酸 (MeJA) 和酸 (SA) 治疗有反应.
结论:
- 这项研究阐明了树中的HbbHLH基因家族,并突出了涉及植物对粉状真菌免疫反应的特定基因.
- 已识别的基因,特别是HbbHLH25,HbbHLH87和HbbHLH162-2,都与素酸和酸信号通路有关.
- 这些发现为培育抗病树品种提供了宝贵的遗传资源和目标.
更多相关视频
06:56Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
12.2K
06:09An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants
Published on: March 9, 2021
3.4K
相关概念视频
Globular and Fibrous Proteins
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Conserved Binding Sites
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...