相关实验视频

Updated: Jan 11, 2026

In Vitro Assay to Study Tumor-macrophage Interaction

In Vitro Assay to Study Tumor-macrophage Interaction

Published on: August 1, 2019

内瘤SPP1+BCL2A1与瘤相关的巨细胞预测对PD1阻塞的反应不佳

Chun-Hao Lai¹, Yu-Ping Hung², Po-Chun Tseng³

¹Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.

Diagnostics (Basel, Switzerland)

|November 13, 2025

概括

需要用于肝细胞癌 (HCC) 免疫治疗的新生物标志物. 研究人员确定SPP1+BCL2A1+TAMs是不良的预后标志物,可能改善ICB治疗反应预测.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
基因组学就是基因组学.

背景情况:

免疫检查点阻塞 (ICB) 对肝细胞癌 (HCC) 有希望,但预测生物标志物有限.
瘤相关巨细胞 (TAMs) 显著影响瘤免疫微环境.
识别特定的TAM子集可以改善对HCC的ICB电阻的理解.

研究的目的:

确定用于预测HCC中ICB反应的新生物标志物.
描述TAMs在HCC瘤微环境中的作用.
调查SPP1和BCL2A1双阳性TAM作为潜在的预后指标.

主要方法:

单细胞RNA测序 (scRNA-seq) 用于分析HCC中的内免疫细胞.
无监督的聚类和基因特征分析确定了SPP1+BCL2A1+TAMs.
使用独立的HCC转录组数据集来验证发现,并与ICB反应相关联.

主要成果:

在HCC患者中发现了增加的内SPP1+BCL2A1+TAMs.
这些TAMs的高表达与ICB治疗的反应较差相关.
这些TAM与不响应者中的免疫抑制微环境和响应者中的细胞毒性T细胞反应有关.

结论:

SPP1+BCL2A1+ TAMs 作为接受ICB治疗的HCC患者的不良预后生物标志物.
这些TAMs促进了免疫抑制的微环境,可能解释了ICB耐药性.
SPP1+BCL2A1+ TAMs 是一个潜在的治疗点,可以提高免疫疗法的疗效.

关键词:

与BCL-2相关的蛋白质A1 这就是PD1 PD1.肝细胞癌是肝细胞癌.分泌的蛋白 1 1 分泌的蛋白与瘤相关的巨细胞.

更多相关视频

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...