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Cristina Alexandra Ciocan¹, Cecilia Bica¹, Liviuta Budisan¹

¹Department of Genomics, MEDFUTURE Institute for Biomedical Research, Iuliu Hațieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania.

International journal of molecular sciences

|November 13, 2025

概括

微RNAs miR-28-5p 和 miR-708-5p 在肺癌中过度表达. miR-708-5p表现出一致的过度表达,这表明它有可能成为肺癌的临床诊断标记物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

肺癌是全球癌症死亡的主要原因之一.
研究像miR-28-5p和miR-708-5p这样的特定微RNA (miRNA) 对于理解肺癌的发病过程至关重要.
识别新的生物标志物和治疗点对于改善患者的治疗结果至关重要.

研究的目的:

研究miR-28-5p和miR-708-5p在肺癌中的作用和表达模式.
分析它们的基因配置和转录因子调节之间的关系.
探索这些miRNAs在肺癌诊断和进展中的潜在临床意义.

主要方法:

从晚期患者的配对瘤和相邻的正常肺组织样本中分析miRNA表达.
使用数据库 (dbDEMC,TransmiR) 进行表达特征分析和转录因子 (TF) -miRNA调节网络分析.
途径丰富分析以确定由这些miRNAs调节的生物途径.

主要成果:

发现miR-28-5p和miR-708-5p都在肺瘤组织中过度表达.
miR-708-5p在肺癌亚型中显示出一致的过度表达,这表明了潜在的诊断价值.
确定了复杂的TF-miRNA调节网络,途径分析将这些miRNA与癌症相关的途径联系起来,例如ECM受体相互作用和Hippo信号传递.

结论:

miR-708-5p在肺癌中呈现一致的过度表达,并具有作为临床诊断生物标志物的潜力.
鉴定的miRNAs调节关键的癌症相关信号通路,有助于肺癌的发展.
对miR-708-5p的进一步研究可能会导致肺癌的新型诊断和治疗策略.

关键词:

生物标志物生物标志物肺癌是一种肺癌.在 miR-28-5p中使用.在 miR-708-5p 中使用.的miRNA种子序列.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...