在冠状动脉疾病中进行签名识别的综合性转录和表观基因组分析:试点研究
Mario Zanfardino1, Anna D'Agostino1, Ilaria Leone1
1IRCCS SYNLAB SDN, 80143 Naples, Italy.
International journal of molecular sciences
|November 13, 2025
概括
这项研究使用多组学来发现冠状动脉疾病 (CAD) 的新分子标记物. 研究人员确定了一种基因特征和监管模式,以改善CAD风险分层.
科学领域:
- 心血管研究研究心血管研究
- 基因组学和表观基因组学
- 分子生物学分子生物学
背景情况:
- 冠状动脉疾病 (CAD) 是全球主要的死亡原因,由动脉样硬化斑块驱动.
- 尽管取得了进展,但~30%的初始CAD事件仍然是致命的,这凸显了早期检测的必要性.
- 有效的风险分层对于管理CAD患者至关重要.
研究的目的:
- 使用多omics方法识别冠状动脉疾病 (CAD) 的新型分子标记物.
- 发现潜在的生物标志物,以改善CAD患者的临床风险分层.
- 在CAD中调查基因表达和染色质可访问性模式.
主要方法:
- 周围血液单核细胞 (PBMC) 的综合转录基因 (RNA-seq) 和表观基因 (ATAC-seq) 分析.
- 来自接受心脏计算机断层扫描血管造影 (CCTA) 患者样本的分析.
- 在独立患者队列中验证关键发现.
主要成果:
- 确定了39个基因,在所有CAD亚型中始终失调.
- 在CAD相关的位置揭示了不同的染色质可访问性模式.
- 证实了关键差异表达基因 (DEGs) 的表达模式,包括Claudin 18 (CLDN18).
结论:
- 多omics数据集成确定了与CAD严重程度相关的核心基因特征.
- 发现了不同的监管模式,为临床风险分层提供了潜在的生物标志物.
- 研究结果支持使用分子标记物来增强CAD管理.
更多相关视频
08:51Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
Published on: September 20, 2024
2.0K
04:41Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
8.6K
相关概念视频
01:30Coronary Artery Disease I: Introduction
863
Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
863
01:30Acute Coronary Syndrome III: Diagnostic Studies
200
Diagnosing acute coronary syndrome or ACS begins with a thorough patient history. Notable symptoms include central, crushing chest pain radiating to the left arm, neck, jaw, or back, along with shortness of breath, sweating (diaphoresis), nausea, vomiting, dizziness, and palpitations.It is crucial to note any history of cardiac illnesses and assess risk factors, including age, gender, smoking, hypertension, diabetes, hyperlipidemia, and a sedentary lifestyle.During physical examination, vital...
200
01:26Coronary Artery Disease II: Pathophysiology
355
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
355
01:30Coronary Artery Disease III: Clinical Manifestations
313
Coronary Artery Disease (CAD) is a primary health risk worldwide, leading to significant morbidity and mortality. The condition arises from the buildup of atherosclerotic plaques within the coronary arteries, resulting in diminished blood supply to the heart muscle.The clinical manifestations of CAD vary widely, from asymptomatic stages to severe, life-threatening conditions. Understanding these manifestations is crucial for early diagnosis and effective management.Angina Pectoris: The Warning...
313