相关实验视频

Updated: Jan 11, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

HC-Pro破坏了miR319-TCP监管途径,以诱导转基因植物的无菌性

Taicheng Jin¹, Weiyan Wang¹, Jiaxue Yu¹

¹The School of Life Sciences, Jilin Normal University, Siping 136000, China.

International journal of molecular sciences

|November 13, 2025

概括

来自烟草静脉带状马赛克病毒的辅助成分蛋白酶 (HC-Pro) 通过改变微RNA和小干扰RNA积累来破坏植物生育能力. 这导致TCP基因的表达增加,影响植物器官的发育,导致植物流产.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

来自烟草静脉带状马赛克病毒 (TVBMV) 的辅助成分蛋白酶 (HC-Pro) 诱导病毒症状和植物流产.
HC-Pro干扰微RNA和小干扰RNA的积累,抵消宿主防御.
在HC-Pro诱导的不孕症中,TEOSINTE分支1/CYCLOIDEA/增殖细胞因子 (TCP) 的作用尚不清楚.

研究的目的:

研究HC-Pro在植物中引起无菌表型的分子机制.
为了确定HC-Pro诱导的堕胎是否与异常的TCP基因表达有关.
阐明病毒蛋白对植物生育调节的影响.

主要方法:

逆转录定量聚合酶链反应 (RT-qPCR) 用于分析基因表达.
北方斑点检测RNA积累的情况.
在烟草和Arabidopsis thaliana模型中使用表达HC-Pro的转基因植物.

主要成果:

在HC-Pro转基因植物中,TCP4和TCP24的表达显著增加.
增加的TCP4表达升高了LIPOXYGENASE2 (LOX2) 的调节,影响了斯酸的合成.
TCP3,TCP4和TCP24基因的异常表达阻止了花和花的延长,以及花粉的释放,导致堕胎.

结论:

HC-Pro影响了针对miR319的TCP基因 (TCP2,TCP3,TCP4,TCP10,TCP24) 的表达.
这种异常基因表达扰乱了正常的花器官发育,导致植物流产.
病毒病原性显著影响作物的生育调节,为植物病毒相互作用提供了洞察力.

关键词:

这就是HC-Pro的特点.这就是TCP的TCP.花的器官发育植物的器官发育斯酸通路的使用 miR31919 在线观看

更多相关视频

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...