精子rDNA复制数和甲基化与男性因素不孕症有关
Alina Michler1, Sarah Kießling1, Jana Durackova1,2
1Institute of Human Genetics, Julius Maximilians University, 97074 Würzburg, Germany.
International journal of molecular sciences
|November 13, 2025
概括
精子rDNA复制数 (CN) 差异很大. 精子rDNA复制数 (CN) 差异很大. 精子中的较低活性rDNA CN与异常的精液参数和降低的怀孕率有关,这表明它在男性不孕症中发挥了作用.
科学领域:
- 生殖生物学 生殖生物学
- 遗传学 遗传学 是一个
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 人类精子中的核糖体DNA (rDNA) 拷贝数 (CN) 是高度可变的.
- 通过促进物低甲基化表示的rDNA的活性对其功能至关重要.
- 在rDNA CN和活动中的个体差异可能会影响男性生育能力.
研究的目的:
- 量化正常与异常精液参数的个体精子中的绝对和可能活跃的rDNA CN.
- 为了研究rDNA CN,促进物甲基化和体外受精 (IVF) /细胞内精子注射 (ICSI) 后的生育结果之间的关联.
- 探索精子rDNA CN在异常男性不孕症中的潜在作用.
主要方法:
- 使用滴滴数字PCR和深度二硫酸盐测序来测量绝对和活性rDNACN.
- 分析了来自190个人的精子样本,根据精液质量和IVF/ICSI怀孕结果分类.
- 评估促进体甲基化水平与rDNA CN和精液参数相关.
主要成果:
- 异常精液参数 (ASP) 样本显示,与正常样本 (NSP) 相比,可能活跃的rDNA CN (104 ± 31) 显着较低 (115 ± 31).
- 在ASP (13.9%) 与NSP (12.1%) 中观察到促进剂甲基化的增加.
- 不导致怀孕的精子样本的绝对值 (225 ± 51) 和活性rDNACN (103 ± 30) 比导致怀孕的精子样本 (分别为249 ± 62和115 ± 31) 低.
结论:
- 精子rDNACN,无论是绝对的还是活跃的,可能是影响异常男性不孕症的关键因素.
- 在常态种子雄性中,较高的活性rDNA CN与成功的妊娠结果相关.
- 大约60个活跃的精子rDNA拷贝似乎足以确定怀孕,这突显了它们在生殖成功中的重要性.
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