综合全转录组分析以阐明参与卵巢发育和绵羊生殖能力差异的circRNA核心调控网络:circRNA2058-miR-9226-5p-MET轴
Bo Gu1, Anqi Wang2, Xinmiao Yu1
1College of Life Science, Jilin Normal University, Siping 136000, China.
Animals : an open access journal from MDPI
|November 13, 2025
概括
研究人员确定了一个关键的分子途径,circRNA2058-miR-9226-5p-MET,它调节了绵羊的卵巢发育和生殖能力. 这一发现为通过有针对性的分子干预来增强绵羊繁殖提供了新的策略.
科学领域:
- 动物基因组学 动物基因组学
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
背景情况:
- 绵羊的卵巢发育和生殖能力受到复杂的监管网络的影响.
- 了解这些网络对于提高绵羊的繁殖效率至关重要,特别是在具有不同生育能力的品种中.
研究的目的:
- 系统地确定候选基因和调节网络,以不同生育能力的羊品种控制卵巢发育.
- 阐明通过多层监管网络来确定母羊生殖能力的机制.
主要方法:
- 来自低生育率 (Ujumqin) 和高生育率 (小尾汉) 羊品种的卵巢的全转录组测序.
- 对竞争的内源RNA (ceRNA) 监管网络的构建和生物信息分析.
- 使用双露西法酶记者基因测试和qRT-PCR验证关键的circRNA-miRNA-mRNA相互作用.
主要成果:
- 基于生育和卵巢发育阶段,构建了两个不同的ceRNA网络.
- 确定了关键的信号通路,包括MAPK,JAK-STAT,WNT,Ras和Hippo.
- 一个核心调节轴,circRNA2058-miR-9226-5p-MET,被确定并验证为卵巢功能的中央调节器.
结论:
- 这项研究解读了控制绵羊卵巢发育的协同调节网络.
- 在羊群中,circRNA2058-miR-9226-5p-MET ceRNA轴被确定为卵泡主导的关键分子开关.
- 这一发现为提高绵羊生殖效率提供了分子基础.
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