延伸器类型和平衡时间是影响火精液解后质量特征的因素
Sara Ataei-Nazari1, Filip Benko1, Tomáš Slanina2
1Institute of Biotechnology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Animals : an open access journal from MDPI
|November 13, 2025
概括
优化火精液冷保存需要仔细选择延长剂和平衡时间. 较短的平衡时间 (20分钟) 总体上改善了解后的精子质量,贝尔茨维尔扩展器显示出更好的结果.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 低温生物学 低温生物学
背景情况:
- 人工授精对于商业火繁殖至关重要.
- 鸟类精子很容易受冷保存损伤的影响,影响生育能力.
研究的目的:
- 调查不同精液延长剂和平衡时间对解后火精子质量的影响.
- 为了确定子精液的最佳冷保存协议.
主要方法:
- 土耳其射精被稀释在贝尔茨维尔,精子流动性中等 (SMM),Botucrio或Kobidil+扩展器中.
- 样品在冷保存和随后解之前平衡了20或40分钟.
- 解后的精子质量使用各种指标进行评估,包括运动性,膜完整性,线粒体功能和DNA完整性.
主要成果:
- 20分钟的平衡期提高了解后的精子运动性,膜完整性,减少了DNA碎片化和活性氧物种 (ROS).
- 贝尔茨维尔扩展器产生了最好的整体精子质量.
- 观察到特定的延长时间相互作用:贝尔茨维尔,博图克里奥和科比迪尔+在20分钟时表现最好,而SMM在40分钟时表现最佳.
结论:
- 扩展器的选择和平衡时间显著影响火精液冷保存的结果.
- 20分钟的贝尔茨维尔扩展器和40分钟的SMM代表了火精子的有效,特定时间的冷保存策略.
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