细胞壁重塑和pH压力协调调节通过转录重编程的莫纳斯克色素生物合成
Xufeng Wang1, Hailei Zhao1, Chengfang Ding1
1State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science & Technology, No. 9, 13th Street, Tianjin Economic and Technological Development Area, Tianjin 300457, China.
Foods (Basel, Switzerland)
|November 13, 2025
概括
改变Monascus purpureus细胞壁可以增强天然色素的产生. 酸性条件和细胞壁压力引发复杂的基因表达变化,揭示了用于工业应用的优化Monascus色素 (MP) 生物合成的途径.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 代谢工程是代谢工程.
背景情况:
- 莫纳斯色素 (MP) 是一种具有传统用途的天然色素.
- 以前的研究将M. purpureus* M9细胞壁结构的改变与MP合成和分泌的增强联系起来.
- 在结合细胞壁应力和酸性条件下对MP生物合成的调节尚未被探索.
研究的目的:
- 为了研究pH压力下MP产生的协调调节和修饰细胞壁多糖.
- 为了在pH值为5.0和3.0的野生型 (M9-WT) 和UDP-galactopyranose突变酶缺乏 (M9-KO) *M. purpureus*菌株中比较基因表达.
- 阐明细胞壁重组和代谢途径在MP生物合成中的作用.
主要方法:
- 使用比较转录学来分析基因表达特征.
- 野生型 (M9-WT) 和淘汰型 (M9-KO) *M.紫色*菌株在pH值5.0和3.0下进行培养.
- 基因和基因组的京都百科全书 (KEGG) 丰富分析和集群的系列测试被用来识别受管制的途径.
主要成果:
- 在pH值为5.0,*MpglfA*淘汰会通过细胞壁重组增强MP分泌,影响67个主要代谢基因.
- 酸性压力 (pH3.0) 在M9-KO和M9-WT中显著增加了差异性表达的基因,诱导了MP前体的降解途径.
- M9-KO表现出增长抑制和酸性应激反应激活用于透适应;MP生物合成基因在高酸度下被抑制.
结论:
- 主要代谢途径,特别是/碳代谢,对MP生物合成进行了关键调节.
- 反对性的调节器ROX1和SPT15调解了对pH反应的转录重编程,影响了MP生物合成.
- 细胞壁组成操纵提供了一种提高MP生产效率的策略.
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