在DSS诱导的结肠炎中Aucubin的保护作用:调节炎症通路,肠道屏障完整性和肠道微生物群
Yong Zhang1, Han Qiao1, Yuxin Cao1
1Institute for Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Foods (Basel, Switzerland)
|November 13, 2025
概括
阿库 (AU) 通过改善肠道屏障功能和减少炎症,有效地缓解小鼠的结肠炎. 这种天然化合物也积极调节肠道微生物群,为炎症性肠道疾病的治疗提供了潜力.
科学领域:
- 药理学和胃肠病学 药理学和胃肠病学
- 自然产品研究自然产品研究
- 微生物组研究 微生物组研究
背景情况:
- 奥库 (Aucubin,AU) 是从Eucommia叶中提取的,它具有已知的抗炎和抗氧化特性.
- 有限的研究存在于AU在治疗大肠炎,一个重要的炎症性肠病的疗效.
- 了解AU对结肠炎的治疗潜力对于开发新型治疗非常重要.
研究的目的:
- 在小鼠模型中研究aucubin (AU) 对硫酸 (DSS) 诱导的大肠炎的治疗作用.
- 评估AU对肠道屏障完整性,炎症标志物和肠道微生物群组成的影响.
- 探索AU在缓解大肠炎中的潜在分子机制.
主要方法:
- 在使用DSS给药的小鼠中建立了结肠炎模型.
- 用于评估其对疾病活动指数 (DAI),器官指数和结肠形态学的影响.
- 通过RT-qPCR分析了抗氧化酶 (SOD,GSH-PX,CAT),脂质过氧化 (MDA),炎症细胞因子 (IL-1β,TNF-α,IL-18) 和肠道屏障基因 (OCLN,CLDN1,CLDN2,ZO-2,MUC1) 的血清和结肠水平.
- 使用16S rRNA测序研究了AU对肠道微生物群组成的影响.
- 进行了体外细胞实验,以评估AU对肠道屏障基因 (MUC2,ZO-1,OCLN,CLDN1),炎症细胞因子 (IL-1β,TNF-α),抗炎因素 (IL-10) 和关键信号通路分子 (IL-1R,MyD88,TAK1,IKKα,RelA) 的影响.
主要成果:
- AU治疗显著降低了DAI得分和器官指数,恢复了结肠形态,并增加了抗氧化酶水平 (SOD,GSH-PX,CAT),同时降低了DSS诱导的大肠炎小鼠的MDA水平.
- AU降低了促炎因素 (IL-1β,TNF-α,IL-18,MyD88,NF-κB) 的表达,并上调了与肠道屏障相关的基因 (OCLN,CLDN1,CLDN2,ZO-2,MUC1).
- AU调节了肠道微生物群,增加了有益的细菌 (Bacteroidota, Firmicutes, Verrucomicrobiota) 并减少了有害的细菌 (Proteobacteria, Deferribacterota).
- 在体外研究证实了AU增强肠道屏障基因表达的能力,减少促炎细胞因子,增加IL-10,并抑制关键炎症信号通路 (NF-κB通路).
结论:
- 奥库 (Aucubin,AU) 通过加强肠道屏障,减少炎症和重新平衡肠道微生物群,在缓解DSS诱导的大肠炎方面显示出显著的治疗潜力.
- AU至少部分通过调节NF-κB信号通路来发挥其保护作用.
- 这些发现为AU作为炎症性肠病治疗的发展提供了坚实的理论和技术基础.
相关概念视频
01:29Drugs for Treatment of Ulcerative Colitis in IBD
439
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
439
01:24Drugs for Peptic Ulcer Disease: Sucralfate as Mucosal Protective Agents
1.3K
In the intricate landscape of the gastric lumen, excessive acid secretion disrupts the natural defense mechanisms, weakening the mucus-bicarbonate barrier. This vulnerability allows pepsin to infiltrate epithelial cells, digesting mucosal proteins and triggering erosion, leading to ulcer formation.
In this scenario, mucosal protective agents like sucralfate play an essential role. Sucralfate, a complex of sulfated sucrose and aluminum hydroxide, demonstrates its usefulness in acidic conditions,...
In this scenario, mucosal protective agents like sucralfate play an essential role. Sucralfate, a complex of sulfated sucrose and aluminum hydroxide, demonstrates its usefulness in acidic conditions,...
1.3K
01:24Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors
1.1K
Peptic ulcer disease, commonly called PUD, represents a multifaceted condition characterized by disruptions in the lining of the gastrointestinal (GI) tract. Central to the protection of the gastrointestinal lining is the mucosal-bicarbonate barrier. This physiological defense mechanism is a formidable shield against the corrosive effects of gastric acid and pepsin secretion in the stomach. Its role is pivotal in maintaining the structural integrity of the stomach's inner lining.
1.1K
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
880
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
880
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
622
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
622