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在癌症分析中检测蛋白质和代谢物通过MALDI 2000-2025

Dorota Bartusik-Aebisher¹, Daniel Roshan Justin Raj², David Aebisher³

¹Department of Biochemistry and General Chemistry, Medical College of The Rzeszów University, 35-310 Rzeszów, Poland.

|November 13, 2025

概括

矩阵辅助激光脱/电离质谱法 (MALDI-MS) 通过绘制瘤中的代谢物图谱,推动了癌症研究. 虽然挑战仍然存在,但MALDI-MSI对精确瘤学和诊断具有前景.

科学领域:

在瘤学瘤学.
分析化学分析化学
生物医学质谱法生物医学质谱法

背景情况:

癌症代谢学提供了关于瘤生物学,生物标志物发现和精确瘤学的见解.
矩阵辅助激光脱/离子化质谱法 (MALDI-MS) 能够通过空间分辨率直接进行组织代谢物分析.

研究的目的:

从2000年到2025年,审查基于MALDI的癌症代谢物检测策略.
突出MALDI-MS在瘤学的技术进步和临床应用.

主要方法:

对用于癌症代谢物分析的MALDI-MS和MALDI-MS成像 (MALDI-MSI) 技术的审查.
讨论技术里程碑,包括先进的矩阵,衍生,MALDI-2和FT-ICR/Orbitrap集成.
对各种癌症类型的临床应用和相关挑战的分析.

主要成果:

马尔迪-MSI能够以接近单细胞分辨率对数千种代谢物的高分辨率映射.
应用包括将瘤与正常组织区分开来,发现生物标志物,绘制异质性和可视化药物代谢.
技术进步提高了灵敏度,代谢物覆盖率和空间保真度.

结论:

MALDI-MS提供与瘤微环境,缺氧和多种癌症治疗反应相关的代谢指纹.
目前面临的挑战包括矩阵干扰,有限的量化,离子抑制,以及缺乏常规诊断的标准化.
未来的方向包括多式成像,机器学习和临床验证位置MALDI-MS用于精密瘤学.

关键词:

癌症代谢物的癌症代谢物.图像质谱仪的质谱仪.矩阵辅助激光脱吸/电离 (MALDI) 技术代谢生物标志物是代谢生物标志物代谢生物组的代谢生物组瘤微环境是一个微环境.

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