确定特定的IgE切割值来预测口服食品中的反应,使用免疫系统对牛奶过敏的挑战
Gökhan Yörüsün1, Ragıp Dere2, Hatice Irmak Celik2
1Department of Pediatric Allergy/Immunology, Ankara Bilkent City Hospital, University of Health Sciences, Ankara, Turkey; gökhan_yrsn33@hotmail.com.
Allergologia et immunopathologia
|November 13, 2025
概括
本研究确定了IMMULITE测量奶牛的特定截止值.
科学领域:
- 过敏和免疫学 过敏和免疫学
- 儿科过敏 儿科过敏
- 诊断测试 诊断测试 诊断测试
背景情况:
- 通过IgE介导的牛奶过敏 (CMA) 的诊断依赖于血清特异性IgE (spIgE),皮肤刺伤测试 (SPT) 和口服食品挑战 (OFC).
- 现有的spIgE截止值数据主要来自ImmunoCAP测定,需要测定特定值才能准确解释.
- IMMULITE是一种常用的免疫测试系统,用于测量临床实践中的spIgE水平.
研究的目的:
- 确定IMMULITE特异性血清特异性IgE (spIgE) 的切割值,可以准确预测IgE介导的牛奶过敏 (CMA) 中的口服食品挑战 (OFC) 结果.
- 在诊断CMA时,建立IMMULITE spIgE测试的可靠参考范围.
- 为了比较IMMULITE spIgE的诊断性能与皮肤刺伤测试 (SPT) 在预测OFC结果.
主要方法:
- 在2019年至2023年期间通过OFC诊断出CMA的50名患者的回顾性分析.
- 使用西门子IMMULITE 2000免疫检测系统测量血清特异性IgE (spIgE).
- 用商业提取物和消毒牛奶进行皮肤刺伤测试 (SPT);使用各种含牛奶的食物进行口服食品挑战 (OFC).
主要成果:
- 开发的IMMULITE特异性截止值:牛奶spIgE的3.13kU/L (AUC:0.776,72%的灵敏度,72%的特异性) 和素的1.85kU/L.
- 已建立的SPT耐受度切线:在巴氏消毒牛奶中为4.25毫米,在商业提取物中为3.5毫米.
- 在84%的阳性病例中,OFC反应发生在酸奶中;26%的反应涉及过敏反应.
结论:
- 这项研究提供了关键的IMMULITE特异性spIgE切割值,用于预测IgE介导牛奶过敏 (CMA) 中的OFC结果.
- 这些发现为使用IMMULITE系统进行CMA诊断的临床医生提供了有价值的参考范围.
- 建立的切线可以帮助更准确,更有效地诊断CMA,在某些情况下可能减少对OFCs的需求.
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