单细胞多基因组学和空间转录基因组学揭示了清细胞细胞癌的转录调节格局
Juan Duan1, Peifeng Ke1, Bangqi Wang2
1Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
这项研究揭示了像YBX3这样的关键基因,通过分析细胞异质性和表观遗传调节,驱动清细胞细胞癌 (ccRCC) 的进展. 结果为精确的ccRCC诊断和治疗提供了洞察力.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 清细胞细胞癌 (ccRCC) 是一种具有高异质性和治疗耐药性的侵袭性癌症.
- 了解ccRCC的细胞和表观遗传环境对于开发有效的治疗方法至关重要.
研究的目的:
- 在ccRCC中全面描述细胞异质性,表观遗传调节和转录因子 (TF) 网络.
- 在ccRCC中识别具有预后和治疗意义的关键基因.
主要方法:
- 集成的单细胞RNA测序 (scRNA-seq),scATAC-seq和空间转录组学 (ST).
- 进行了单细胞伪药和基因组丰富分析 (GSEA).
- 实验验证了Y盒结合蛋白3 (YBX3) 的功能.
主要成果:
- 在ccRCC瘤微环境 (TME) 中确定了16种不同的细胞群.
- 揭示了细胞类型特定的染色质可访问性和涉及的关键TFs (HNF1B,FOS-JUNB).
- 发现了五个预后基因 (YBX3,CUBN,SNHG8,ACAA2,PRKAA2) 与代谢重编程和ccRCC进展有关;YBX3敲击抑制了增殖和迁移.
结论:
- 阐明了ccRCC的细胞异质性,表观遗传景观和关键驱动基因.
- 多omics数据集成为精确的ccRCC诊断和治疗提供了洞察力.
- 强调了YBX3在ccRCC进展中的关键作用.
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