全血免疫代谢特征与外围血液单核细胞免疫代谢特征的比较
Ibrahima Diallo1,2, Graham Heieis1, Mikhael D Manurung1
1Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
Immunometabolism (Cobham, Surrey)
|November 13, 2025
概括
全血 (WB) 为免疫代谢分析提供了对外围血液单核细胞 (PBMC) 的实用替代方案,在免疫细胞子集和代谢状态上显示出高度相似性. 然而,在某些细胞类型中存在特定的代谢标记差异.
科学领域:
- 免疫代谢过程中的免疫代谢.
- 细胞的新陈代谢
- 免疫反应调节 免疫反应调节
背景情况:
- 周围血液单核细胞 (PBMC) 是免疫代谢分析的标准,但需要大量的血液和复杂的处理.
- 全血 (WB) 提供了一个不那么具有挑战性的替代方案,但其与PBMCs在代谢表征方面的可比性尚未确立.
研究的目的:
- 为了比较全血 (WB) 和外围血液单核细胞 (PBMC) 的免疫代谢概况.
- 为了确定WB是否是PBMCs的可行的替代品,用于免疫代谢研究.
主要方法:
- 从六名健康的捐赠者那里收集了配对的WB和PBMC样本.
- 确定了免疫细胞种群,并使用光谱流细胞计评估了它们的代谢状态.
- 在CryoStor®-CS10介质中收集WB;通过Ficoll密度梯度分离PBMC.
主要成果:
- 在WB和PBMC之间的免疫细胞子集频率和代谢概况中观察到很高的相似性.
- 在特定的代谢标记物中发现了差异:来自PBMC的CD8+TEMRA,NKT和NK细胞中较高的葡萄糖载体1;来自WB的天真CD4+T细胞中较高的ATP5a.
结论:
- 全血 (WB) 为广泛的免疫代谢概况提供了一个潜在的替代PBMCs.
- 在比较WB和PBMC之间针对特定免疫细胞子集的免疫代谢数据时,建议谨慎.
更多相关视频
07:40Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021
18.3K
06:26Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
Published on: March 28, 2025
921
相关概念视频
01:28Cells of the Innate Immune Response
8.4K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.4K
01:20Special Features of Adaptive Immunity
3.7K
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
3.7K