微生物培养与mNGS:周围假肢关节感染的诊断变异
Lan Lin1,2,3, Xiaolin Li1,2,3, Jiayu Li1,2,3
1Department of Orthopedics, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
Frontiers in cellular and infection microbiology
|November 13, 2025
概括
与传统的微生物培养相比,甲基因组下一代测序 (mNGS) 在周围假肢关节感染 (PJI) 中提供了改进的病原体检测. 以前的抗生素使用和多微生物感染增加了诊断差异.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 周围假肢关节感染 (PJI) 在骨科手术中构成了重大挑战.
- 准确的病原体鉴定对于有效的PJI治疗至关重要.
研究的目的:
- 为了比较微生物培养和基因组下一代测序 (mNGS) 的诊断性能,用于PJI.
- 确定影响这些诊断方法之间的差异的因素.
主要方法:
- 对167名疑似PJI的患者进行了回顾性分析,这些患者正在接受修复手术.
- 微生物培养和mNGS从关节液,组织或超声波液样本的结果进行比较.
- 统计分析以确定不一致结果的风险因素.
主要成果:
- 以前的抗生素使用,多微生物感染,罕见的病原体和手术内组织样本是不一致的风险因素.
- 发现了负的微生物培养与积极的mNGS发现.
- 一致的样本类型是防止不一致的保护因素.
结论:
- 临床医生应量身定制诊断策略,将mNGS与微生物培养相结合.
- 优化样本采集,包括使用来自同一解剖部位的组织,提高准确性.
- 这种方法可以提高病原体的识别和治疗的有效性,尽管潜在的成本增加.
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