基因组和酒精脱酶数据集的草稿 热氧核细菌uzonensis细菌菌株AK85的基因组和酒精脱酶数据集
Clay A Abraham1,2, Eva Maria Ingvadottir3, Kevin M Bradley1
1Foundation for Applied Molecular Evolution, Alachua, FL, USA.
Data in brief
|November 13, 2025
概括
从冰岛温泉中分离的Thermoanaerobacter uzonensis AK85菌株的基因组草案揭示了其生产酒精和其他生物制造产品的潜力. 这项研究详细介绍了其代谢酶和酒精脱酶活性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物技术是生物技术.
背景情况:
- 热氧细菌uzonensis菌株AK85是一种热爱性,厌氧细菌.
- 众所周知,Thermoanaerobacter属的成员可以从各种基板生产酒精.
研究的目的:
- 提出Thermoanaerobacter uzonensis菌株AK85.5的基因组序列草案.
- 分析基因组数据集的生物技术潜力,特别是在酒精生产中.
- 为了研究其酒精脱酶的活性.
主要方法:
- 基因组测序使用MGISEQ-2000与150bp配对结尾库.
- 使用Prokka进行基因组组装和注释.
- 通过对NAD+和NADP+进行色度测试,分析酒精脱酶.
主要成果:
- 组装的基因组为2,577,794个基因组,其GC比为33.69%.
- 鉴定了41种参与碳水化合物,氨基酸和酸代谢的酶.
- 原生酒精脱酶证明了氧化长链初级酒精的能力,如1-octanol和醇.
结论:
- 基因组数据证实了Thermoanaerobacter uzonensis AK85菌株对酒精和生物产品生成的生物技术能力.
- 该研究提供了关于其酒精脱酶的辅因子和基质特异性的见解.
- 基因组序列在NCBI数据库中公开提供.
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