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CRISPy-web 3.0:一个统一的平台,用于用于CRISPR和TnpB基因组编辑应用的多模导向RNA设计

Sihan Yang¹, Zupeng Cai¹, Natalie Chia¹

¹State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Synthetic and systems biotechnology

|November 13, 2025

概括

CRISPy-web 3.0通过为CRISPR-Cas9,CRISPR干扰和TnpB系统提供多功能指导RNA设计来增强基因组编辑. 这个更新的平台提高了更广泛的 prokaryotic 基因组编辑应用程序的效率和特异性.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

克里斯普尔-Cas9技术彻底改变了基因组编辑.
有效和特定的引导RNA (gRNA) 设计对于成功的基因组编辑至关重要.
现有的工具往往只专注于正规的CRISPR-Cas9系统.

研究的目的:

介绍CRISPy-web 3.0,这是一个用于合理指导RNA设计的升级平台.
扩展CRISPR-Cas9之外的功能,包括CRISPR干扰 (CRISPRi) 和TnpB/ωRNA系统.
为基因组编辑应用提供改进的用户界面和增强的后端可扩展性.

主要方法:

开发一个互动的基于Web的平台,CRISPy-web 3.0.0.
整合对多个基因组编辑系统 (Cas9,CRISPRi,TnpB/ωRNA) 的支持.
实施gRNA效率和特异性的评分系统,考虑不匹配容忍,位置上下文和PAM要求.

主要成果:

CRISPy-web 3.0提供了一个重新设计的界面,具有多种编辑模式和可视化选项.
该平台支持选择目标区域 (ORF,5' UTR) 并可视化链方向,非目标和突变结果.
评分系统评估CRISPR-Cas9应用的gRNA性能,提高合理的设计.

结论:

CRISPy-web 3.0 是用于指导RNA设计的全面和可扩展的平台.
升级版可以在更广泛的 prokaryotic 系统中进行基因组编辑.
该平台为各种基因组编辑工具 (包括CRISPR和TnpB) 提供了合理的gRNA设计.

关键词:

克里斯普里是什么?克里斯普里是什么?基因组编辑基因组编辑指南RNA指南RNA是指导RNA的指南RNA.TnpBB 在线阅读网络服务器网络服务器

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