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相关概念视频

Open Angle Glaucoma: Treatment01:27

Open Angle Glaucoma: Treatment

In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...

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相关实验视频

Updated: Jun 21, 2026

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
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甲状腺下注射体积与猪眼的持续外视网膜稀释有关.

Immanuel P Seitz1,2,3, Ruslan Nyshchuk4,5, Zdenka Ellederova4

  • 1Oxford Eye Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom.

Investigative ophthalmology & visual science
|November 13, 2025
PubMed
概括

视网膜下注量与猪眼中的外视网膜稀释有关. 减少注射量可以减轻视网膜稀疏,并提高临床前治疗的安全性.

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科学领域:

  • 眼科医生 眼科 眼科
  • 临床前研究 临床前研究
  • 基因疗法交付 基因疗法交付

背景情况:

  • 转基因猪对于临床前基因和细胞疗法测试至关重要.
  • 视网膜下注射 (SRI) 是一种常见的输送方法,但可能导致视网膜变薄.
  • 了解SRI对视网膜结构的影响对于治疗开发至关重要.

研究的目的:

  • 在猪眼中调查子视膜注射量 (SRV) 与外视网膜稀释之间的相关性.
  • 为了比较平衡盐溶液 (BSS) 和腺相关病毒 (AAV) 注射对视网膜厚度的影响.
  • 在一个临床前模型中评估子皮注射的安全性.

主要方法:

  • 十个转基因猪眼接受了BSS或AAV的升级SRV (50-200μL).
  • 外视网膜厚度 (ORT) 变化使用光学连贯性断层扫描 (OCT) 量化,注射后六个月.
  • 组织学证实了OCT发现的视网膜稀释在血栓区域内.

主要成果:

  • 在SRV和外视网膜稀释 (ORT损失) 之间观察到显著的线性相关性 (R2 = 0.73).
  • 每增加9μL的SRV就导致大约1μm的ORT损失.
  • 在最大体积 (200μL) 的AAV注射显示出比BSS更大的稀释.

结论:

  • 在猪眼中,SRV是导致外视网膜变薄的重要因素.
  • 将SRV降至每小时100μL以下,可以减少体积应力和视网膜变薄.
  • 优化手术技术可以提高通过SRI提供的基因和细胞疗法的临床前安全性.