使用UHPLC-TOF-MS和UHPLC-QTRAP-MS/MS验证一种用于植物性肉类替代品的多菌毒素检测方法,并将其应用于商业化植物性汉堡样本
Paula Llorens Castelló1, Marta Leite2,3, Andreia Freitas2,3
1Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Burjassot, Spain.
概括
植物性肉类替代品 (PBMA) 可能含有有害的真菌毒素. 这项研究开发了一种敏感的方法来检测PBMA中的20种真菌毒素,在商业产品中发现了几种真菌毒素,突出了持续安全监测的必要性.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 食品安全 食品安全
背景情况:
- 植物性肉类替代品 (PBMA) 作为可持续的食物来源的需求日益增长.
- 由于谷物,豆类和坚果等成分,PBMA中存在潜在的真菌毒素污染风险.
- 需要可靠的分析方法来确保PBMA的安全性.
研究的目的:
- 验证分析工作流程,用于检测PBMA中的20种真菌毒素.
- 根据欧盟监管标准评估方法的性能.
- 为了确定商业PBMA产品中的真菌毒素的存在和含量.
主要方法:
- 优化基于QuEChERS的提取方法用于样品制备.
- 超高性能液态色谱与飞行时间质谱学 (UHPLC-TOF-MS) 结合.
- 超高性能液态色谱与三重四极质谱仪 (UHPLC-QTRAP-MS/MS) 结合使用.
主要成果:
- 经过验证的方法显示出高灵敏度,定量限值 (LOQ) 低于监管限值.
- 在12个PBMA汉堡样本中检测到包括青毒素 (B1,B2), moniliformin 和 tentoxin 在内的真菌毒素.
- 检测到的真菌毒素水平在0.3到103 ng g-1.1之间.
结论:
- 开发的分析工作流适合在PBMA中可靠地确定真菌毒素.
- 菌毒素污染是PBMA市场的一个重要问题.
- 对植物性肉类替代品中的真菌毒素的持续监测对于消费者安全至关重要.
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