泛癌单细胞RNA测序分析细化了多来源的单细胞和巨细胞系
Truc Do Thanh Nguyen1, Andrew J Lee2, Hyun Jung Park3
1Sungkyunkwan University, Suwon, Korea (South), Republic of.
Cancer immunology research
|November 13, 2025
概括
与瘤相关的巨细胞 (TAMs) 有不同的起源,影响癌症的进展. 识别TAM亚型为改善患者的治疗结果和免疫治疗反应提供了新的治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 瘤相关巨细胞 (TAMs) 是瘤微环境的关键调节者,影响癌症进展,免疫逃避和血管形成.
- 了解TAM异质性对于开发有效的癌症疗法至关重要.
研究的目的:
- 使用单细胞转录组学,在健康和癌症组织中创建一个全面的髓状细胞地图.
- 调查不同的TAM子集的起源和功能作用.
- 探索TAM异质性对患者预后和免疫治疗反应的影响.
主要方法:
- 骨髓状细胞群的单细胞转录组学分析.
- 在健康和胰腺癌组织之间进行比较分析.
- 特定的TAM和骨髓原抑制细胞 (MDSC) 亚型的识别和表征.
主要成果:
- 在TAM种群中发现了显著的异质性,表明双重起源:居住组织巨细胞 (C1QC+ TAM) 和循环单细胞 (SPP1+ TAM,ISG15+ TAM).
- 确定THBS1+ MDSC及其SPP1+ TAM后代是瘤进展,免疫抑制和血管生成的关键驱动因素.
- 建立了一个二分化的TAM模型:C1QC+ TAM与更好的结果相关,而THBS1+ MDSCs-SPP1+ TAMs系系与较差的生存率和免疫疗法耐药性有关.
结论:
- TAMs表现出不同的起源和功能状态,影响癌症动态.
- THBS1+ MDSCs-SPP1+ TAMs轴代表了一个亲瘤源的途径.
- 针对特定的TAM子集有望改善癌症治疗策略和免疫疗法疗效.
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