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下一代测序对骨科相关传染病的高诊断价值:对24项队列研究的定量分析

Mingjiang Luo¹, Zubing Mei^2,3, Min Xu⁴

¹Department of Spine Surgery, Lishui Hospital of Wenzhou Medical University, Lishui People's Hospital, Lishui, Zhejiang, China.

International journal of surgery (London, England)

|November 13, 2025

概括

下一代测序 (NGS) 在诊断骨科感染方面显示出高精度,优于传统方法. 这一元分析证实了NGS是识别骨和关节感染病原体的可靠工具.

科学领域:

整形外科整形外科整形外科
传染性疾病传染性疾病
分子诊断学分子诊断学

背景情况:

诊断骨科感染的传统培养方法缓慢,错误阴性率高.
下一代测序 (NGS) 为微生物诊断提供了更快,更全面的方法.
对于骨科感染,NGS的诊断准确性需要进一步评估.

研究的目的:

进行元分析,评估NGS对骨科相关传染病的诊断准确性.
为了比较NGS与传统诊断方法的性能.

主要方法:

在PubMed,Embase和Cochrane数据库中进行系统的文献搜索,截至2025年1月.
包括24项分析NGS用于骨科感染诊断的研究.
计算聚合的灵敏度,特异性,概率比率和诊断几率比率 (DOR).
评估整体诊断准确度,使用总结接收器操作特征 (SROC) 曲线和曲线下的面积 (AUC).

主要成果:

聚合灵敏度:0.88 (95% CI:0.84-0.92); 聚合特异性:0.94 (95% CI:0.90-0.96). 聚合特异性:0.94 (95% CI:0.90-0.96) 聚合特异性:0.88 (95% CI:0.84-0.92); 聚合特异性:0.94 (95% CI:0.90-0.96); 聚合特异性:0.94 (95% CI:0.90-0.96); 聚合特异性:0.94; 聚合特异性:0.94 (95% CI:0.90-0.96).
阳性概率比 (PLR): 14.5; 阴性概率比 (NLR): 0.12; DOR: 118.5; 阳性概率比 (PLR): 14.5; 阴性概率比 (NLR): 0.12; 阴性概率比 (DOR): 118.
SROC曲线下的面积 (AUC) 为0.97 (95% CI:0.95-0.98),表明诊断的准确性很高.
没有发现显著的出版偏差 (p=0.24).

结论:

在多种样本,平台和区域中,NGS证明了对骨科感染的高诊断准确性.
研究之间的显著异质性需要谨慎地解释结果.
未来的研究应该专注于大规模的前性研究和直接比较,以标准化NGS临床实施.

关键词:

没有NGS,没有NGS.队列研究是一项队列研究.这是下一代测序.与骨科相关的传染病.定量分析量化分析

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