探索肠道微生物群及其在肺结核中的预测功能:使用公共16S数据集的多区域研究
Tejaswini Baral1, Anwesh Maile2, Nagarajaram Hampapathalu Adimurthy2
1Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
PloS one
|November 13, 2025
概括
抗结核疗法显著改变了肠道微生物的多样性和功能在肺结核患者,特别是在西非. 地理位置对微生物多样性的影响比疾病本身更大.
科学领域:
- 微生物组研究的研究.
- 传染病流行病学 传染病流行病学
- 肠道健康和微生物生态学
背景情况:
- 由Mycobacterium tuberculosis引起的肺结核 (PTB) 是一个重要的全球卫生问题,不成比例地影响着发展中国家.
- 这项研究研究了肠道微生物对PTB的反应及其使用16S amplicon测序数据的治疗.
研究的目的:
- 分析抗结核治疗对PTB患者肠道微生物多样性和功能的影响.
- 为了比较不同地理位置的微生物组变化.
主要方法:
- 利用微生物生态学的定量洞察 v.2 (QIIME 2) 用于微生物多样性分析.
- 通过对未观察到状态的重建来对社区进行人科遗传调查 v.2 (PICRUSt 2) 用于功能性路径预测.
- 分析了来自四个地理区域的公开可用的16S安普利康测序数据集.
主要成果:
- 在西非PTB患者中,经过两个月的抗结核治疗后,观察到肠道微生物α多样性的统计显著下降.
- 在接受治疗的PTB患者中发现预测的微生物代谢途径 (包括维生素和氨基酸生物合成和能量生产) 的耗尽.
- 在地点内对对比或在总体β多样性中没有发现显著差异.
结论:
- 抗结核疗法显著影响肠道微生物多样性和预测功能,可能需要更长时间的治疗来加剧这些变化.
- 地理位置在塑造微生物多样性方面发挥的作用比疾病状态更为关键.
- 研究结果表明,精准医学方法有可能基于个人或区域微生物组概况来个性化干预.
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