在废水中测量登革热病毒载荷的方法方法方法:系统审查和元分析议定书
Nurul Amalina Khairul Hasni1, Sakshaleni Rajendiran1, Nurul Athirah Naserrudin2
1Environmental Health Research Center, Institute for Medical Research Malaysia, National Institutes of Health, No. 1, Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, Shah Alam, 40170, Malaysia, 60 0333627443 ext 7443.
JMIR research protocols
|November 13, 2025
概括
基于废水的监测 (WBS) 提供了早期登革热检测,但方法需要标准化. 本系统性审查评估了废水中目前的登革热病毒 (DENV) 检测技术,以改善公共卫生战略.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生 公共卫生
- 病毒学 病毒学
背景情况:
- 登革热病毒 (DENV) 的出现构成了全球公共卫生挑战.
- 临床监测在早期发现疫情方面存在局限性.
- 基于废水的监测 (WBS) 显示出积极的公共卫生干预措施的前景.
研究的目的:
- 系统地审查和确定目前在废水中检测DENV的方法.
- 确定DENV WBS中的差距和最佳实践.
主要方法:
- 按照PRISMA指南在主要数据库 (PubMed,Embase,Scopus,Web of Science) 进行系统的文献搜索,直到2025年6月.
- 提取关于采样技术,检测方法,研究地点和血清型的数据.
- 使用ROBINS-I和GRADE工具进行质量评估.
- 对DENV WBS方法的描述性分析和元分析.
主要成果:
- 对废水中DENV检测数据的综合,包括采样和病毒检测方法.
- 对研究地点,地理分布和确定的登革热血清型进行分析.
- 进行元分析以评估方法,考虑异质性.
结论:
- 本系统审查协议概述了评估DENV WBS方法的综合方法.
- 结果将为登革热监测提供最佳实践,并指导未来的研究.
- 这项研究旨在提高WBS在登革热爆发预测方面的可靠性和有效性.
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