相关实验视频

Updated: Jan 11, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

端粒长度和端粒酶基因表达的差异与年龄以及太平洋组织之间的差异

F Akcha¹, R Le Roux¹, C Roman¹

¹Ifremer Centre Atlantique, Chemical Contamination of Marine Ecosystems (CCEM) research unit, Nantes, France.

Marine environmental research

|November 13, 2025

概括

这项研究优化了中的端粒长度 (TL) 测量,用于生物监测. 我们发现TL随着年龄的增长而下降,并因组织而异,为压力影响研究提供了一个框架.

科学领域:

海洋生态毒理学海洋生态毒理学
生物标志物的发展发展.
分子生物学分子生物学

背景情况:

海洋生物面临化学压力因素,需要可靠的生物标志物用于生物监测计划.
端粒长度 (TL) 是累积压力的潜在生物标志物,但其在生态毒理学中的使用受到测量挑战和对其动态的理解不足的阻碍.
太平洋 (Crassostrea gigas) 是用于海洋环境监测的哨兵物种.

研究的目的:

开发和优化一种可靠的方法来测量太平洋的端粒长度 (TL),Crassostrea gigas.
为了研究中不同组织和年龄类别TL的变化.
探索端粒动力学与中的端粒酶基因表达之间的关系.

主要方法:

对比DNA提取方法,从冷的组织中分离高分子量DNA.
优化了定量PCR (qPCR) 试验,以准确量化TL.
评估了跨组织和年龄类别的TL变异,并使用RT-qPCR分析了端粒酶基因表达.

主要成果:

在冷的组织中建立了一个强大的DNA提取和TL测量协议.
观察到TL的显著组织特异性差异,消化腺显示最短的端粒.
在所有组织中检测到与年龄相关的TL持续下降,成人中端粒酶基因表达较低.

结论:

开发的方法为生态毒理学研究中的TL评估提供了可靠的工具.
中的端粒长度受组织类型和年龄的影响,反映了累积的生物过程.
这些发现为在生物监测计划中使用TL作为环境压力的生物标志物提供了一个框架.

关键词:

提取DNA 提取DNA的方法生态毒理学生态毒理学一个的.泰隆玛酶的使用方法泰洛米尔 (Telomere) 是一种电离体.qPCRR 是一个很好的方法.

更多相关视频

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Published on: March 22, 2024

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Published on: December 5, 2025

相关概念视频

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...

Telomeres and Telomerase

Telomeres and Telomerase

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...

The Effect of Aging on Tissues

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...