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基于选择性减弱黄金纳米集群的过氧化酶活性,对硫醇进行可视化和度检测

Meiling Li¹, Nanping Hong¹, Huaidong Peng²

¹School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China; Key Laboratory of State Administration of TCM for Digital Quality Evaluation of Chinese Materia Medica, Guangzhou 510006, China; Engineering & Technology Research Center for Chinese Materia Medica Quality of Guangdong Province, Guangzhou 510006, China.

Journal of pharmaceutical and biomedical analysis

|November 13, 2025

概括

本研究提出了一种使用金纳米集群检测硫醇 (SFX) 的新方法. 该方法具有敏感性,选择性,并允许在动物样本中可视检测SFX.

科学领域:

纳米材料科学科学纳米材料科学
分析化学分析化学
生物医学工程生物医学工程

背景情况:

硫醇 (SFX) 是一种需要敏感检测方法的抗生素.
牛血清白蛋白 (BSA) 稳定黄金纳米集群 (BSA-AuNCs) 表现出类似过氧化酶的活性.
现有的SFX检测方法可能缺乏灵敏度或选择性.

研究的目的:

开发一种简单,灵敏和选择性的SFX检测方法.
为了利用SFX对BSA-AuNCs催化活性的抑制作用.
为了使SFX的色度和度检测.

主要方法:

BSA稳定黄金纳米集群 (BSA-AuNCs) 的合成.
用BSA-AuNCs对基于过氧化 (H2O2) 的o-phenylenediamine (OPD) 氧化的催化.
对BSA-AuNCs的催化活性进行SFX抑制的研究.
使用紫外线光谱,裸眼观测和度测量进行检测.

主要成果:

BSA-AuNCs催化了OPD氧化,产生了一种黄色的颜色.
SFX显著抑制了BSA-AuNCs的催化活性.
已达到检测极限:7nM (紫外线),10nM (肉眼) 和0.3nM (度).
该方法在动物肉和血液样本中检测SFX的成功应用.

结论:

一种基于BSA-AuNCs的SFX检测的新有效方法已经建立.
开发的方法为SFX提供了高灵敏度和选择性.
这种方法为监测食品安全和兽医应用中的SFX提供了有价值的工具.

关键词:

类似过氧酶的活性.苏尔菲索克萨 (sulfisoxazole) 是一种硫化物.硫胺类药物是一种硫胺类药物.视觉检测视觉检测是一种视觉检测.黄金纳米集群的金色纳米集群

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