通过赖特-吉姆萨染色改善细菌涂抹检测:临床微生物学实验室的多中心研究
Pengyu Ji1, Shuqiao Yuan1, Tingting Zeng2
1Department of Laboratory Medicine, the 1(st) School of Clinical Medicine, Lanzhou University; the First Hospital of Lanzhou University, Lanzhou, 730000, Gansu Province, China.
Journal of microbiological methods
|November 13, 2025
概括
赖特-吉姆萨染色显著改善了唾液中的细菌检测和积极的血液培养 (PBCs) 错过了格拉姆染色. 这种方法减少了错过的诊断,特别是在初级医院,提高了微生物学实验室的准确性.
科学领域:
- 微生物学 微生物学
- 临床诊断 临床诊断 临床诊断
- 染色技术 染色技术
背景情况:
- 格拉姆染色是一种用于细菌检测的标准显微镜技术,但具有局限性.
- 在唾液和阳性血液培养 (PBCs) 中错过的细菌检测可能导致诊断延迟和治疗失败.
- 需要改进的染色方法来提高细菌识别的准确性.
研究的目的:
- 为了评估赖特-吉姆萨染色在改善细菌检测中的有效性,与格拉姆染色相比.
- 评估赖特-吉姆萨染色对减少临床样本中遗漏的细菌检测率的影响.
- 分析格拉姆染色失踪并通过赖特-吉姆萨染色检测到的微生物类型.
主要方法:
- 一项前性多中心研究,涉及5667个液样本和5502个PBC.
- 赖特-吉姆萨染色是通过初始格拉姆染色对细菌负的标本进行的.
- 错过检测病例的分析,未检测到的微生物类型,以及初级和中央医院之间的比较.
主要成果:
- 赖特-吉姆萨染色在3.0%的格拉姆染色阴性唾液样本和42.0%的格拉姆染色阴性PBC中发现了细菌.
- 在唾液样本中发现了321种以前未被检测到的微生物形式.
- 主要污染物包括凝固酶负型葡萄球菌和Micrococcus spp.
- 与区域中心医院相比,初级医院的错误检测率明显高 (p < 0.001).
结论:
- 赖特-吉姆萨染色实质性地降低了涂抹和PBC中遗漏的细菌检测率.
- 这种染色方法提高了微生物学实验室的细菌检测,特别是对于格拉姆染色负结果.
- 结合赖特-吉姆萨染色可以帮助协调实验室间的差异,并提高诊断准确性,特别是在资源有限的环境中.
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