蒸废谷物的SSCF生物转化为增强单细胞蛋白质生产的多阶段优化
Shu Jiang1, Yuan Peng1, Hongye Shen1
1National Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Bioresource technology
|November 13, 2025
概括
这项研究开发了一种用于从蒸废谷物 (DSG) 制造可持续单细胞蛋白 (SCP) 的新方法. 综合方法有效地将纤维纤维素废物转化为高蛋白质的SCP,解决蛋白质短缺和废物回收问题.
科学领域:
- 生物技术是生物技术.
- 可持续的生物制造
- 废弃物价值化 废弃物价值化 废弃物价值化
背景情况:
- 蒸废谷物 (DSG) 是一种具有生物处理潜力的纤维素类废物流.
- 蛋白质短缺需要可持续和高效的蛋白质生产方法.
- 像DSG这样的废物流的价值化与循环经济原则保持一致.
研究的目的:
- 从蒸废谷物 (DSG) 制造高价值单细胞蛋白 (SCP) 的综合工艺.
- 为了优化预处理,酶解水解和发酵,以便酵母有效地共同利用糖.
- 验证开发的SCP生产过程的可扩展性和效率.
主要方法:
- 用稀释硫酸辅助的高温预处理,然后进行酶性水解.
- 利用协同的β-葡萄糖酶和葡萄糖胺酶系统来增强糖分的释放.
- 采用高性能酵母菌株 (Cyberlindnera jadinii R2878) 进行和六的联合代谢.
- 实施了与喷雾干燥相结合的同时糖化和共发酵 (SSCF).
主要成果:
- 在酶性水解过程中实现了92.42%的总糖释放.
- 生产的单细胞蛋白 (SCP) 含有47.5%的原蛋白 (CP) 和14.2%的酸溶性蛋白 (ASP).
- 获得了0.94g/L/h的高生产率.
- 试点规模验证 (50 L) 证实了工艺的稳定性和可扩展性.
结论:
- 综合过程为DSG高价值回收到SCP提供了一个创新的和可行的途径.
- 先进的预处理,强大的酵母新陈代谢和SSCF喷雾干燥的结合提高了下游加工效率.
- 这种方法为蛋白质生产和纤维素废物利用提供了可持续的解决方案.
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