原纤维的双重定位用于指导3D打印结构中的细胞对齐
Diya Singhal1, Fotis Christakopoulos2, Lucia G Brunel1
1Stanford University, Department of Chemical Engineering, Stanford, CA, USA.
Acta biomaterialia
|November 13, 2025
概括
研究人员开发了一种3D打印方法,以创建具有图案的原纤维对齐的仿生支架. 这种技术精确地控制纤维的方向,指导组织工程应用的细胞行为.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 3D打印技术的使用
背景情况:
- 自然组织表现出复杂的纤维蛋白对齐,对其功能至关重要.
- 现有的制造方法通常只能实现单向纤维对齐.
- 模仿本地组织架构需要先进的脚手架制造技术.
研究的目的:
- 开发一种可重复的3D打印方法,用于制造具有两个独立方向的纹理纤维对齐的仿生支架.
- 通过基于挤出的3D打印来研究对原纤维对齐的控制.
- 为了证明这些支架引导细胞对齐的能力.
主要方法:
- 基于挤出的3D打印,可控制印刷丝的横向扩散.
- 操纵打印头运动和油墨挤出速度之间的速度比.
- 使用原生物材料墨水用于脚手架制造.
- 在印刷的支架上播种人类角膜介质层细胞.
主要成果:
- 通过控制发光线的扩散,实现与流动方向平行或垂直的图案纤维对齐.
- 对影响光纤方向的打印参数的验证理论预测.
- 证明了与空间定义的平行和垂直的原纤维对齐区域的支架的制造.
- 观察到种子细胞采用了与底层的原纤维模式保持一致的形态.
- 在空气打印和嵌入式3D打印中成功地应用了该技术.
结论:
- 开发了一个理论和实验框架,用于可重复制造3D打印结构的可控原纤维图案.
- 这种技术可以创建复杂的支架,模仿本地组织的异构结构.
- 有图案的支架有效地引导细胞对齐,显示了先进组织工程应用的潜力.
相关概念视频
01:13Cell-matrix's Response to Mechanical Forces
3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
3.4K
01:56Structural Protein Function
29.7K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
29.7K
01:11Fibril-associated Collagen
3.2K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
3.2K
01:13Collagens are the Major Structural Proteins of ECM
5.6K
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. Collagen fiber is made from fibrous protein subunits linked together to form a long, straight fiber. Collagen fibers, while flexible, have great tensile strength, resist stretching, and give ligaments and tendons their characteristic resilience and strength. These fibers hold connective tissues together, even during the body's movement.
Connective tissue proper includes loose...
Connective tissue proper includes loose...
5.6K