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qMaLioffG:一种基因编码的绿色光生命周期指标,可用于细胞内ATP的定量成像

Satoshi Arai¹, Hideki Itoh², Cong Quang Vu³

¹WPI Nano Life Science Institute, Kanazawa University, Kanazawa, Japan. satoshi.arai@staff.kanazawa-u.ac.jp.

Nature communications

|November 13, 2025

概括

研究人员开发了一种新的遗传编码指标,qMaLioffG,用于使用光终身成像显微镜测量腺三酸盐 (ATP) 水平. 这种工具可以在细胞和组织中进行定量ATP成像,揭示ATP的空间分布.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
分子成像学分子成像学

背景情况:

使用光寿命变化的基因编码指标为代谢物和信号分子提供了先进的定量成像能力.
这些指标克服了基于强度的方法的局限性,减少了来自度变化,细胞变化和焦点漂移的工件.
光寿命指标的稀缺性,特别是那些与标准的488nm激光器兼容的指标,阻碍了它们的广泛采用.

研究的目的:

引入一种新的,单一的绿色光蛋白基腺三酸盐 (ATP) 指标,qMaLioffG,用于定量光终身成像.
证明该指标在测量各种细胞区和多细胞系统中生理相关的ATP度方面的实用性.

主要方法:

开发了qMaLioffG,这是一个基于单个绿色光蛋白的基因编码指标.
根据生理ATP度对指标的光寿命变化 (1.1 ns) 的表征.
在多种细胞类型的细胞质和线粒体以及多细胞Drosophila大脑和HeLa细胞球体中使用qMaLioffG进行定量ATP成像的应用.

主要成果:

qMaLioffG表现出显著的光寿命转移,使得精确的ATP度检测.
在稳定状态条件下,在各种细胞类型中实现了细胞质和线粒体中ATP水平的定量成像.
在多细胞系统中发现了空间异质的ATP水平,包括Drosophila大脑和HeLa细胞球体.

结论:

qMaLioffG提供了一种有价值的工具,用于使用标准显微镜进行定量,空间分辨率的ATP成像.
该指标与488nm激光器的兼容性及其在多细胞模型中的性能扩大了研究细胞能量代谢的潜力.
这项工作有助于更深入地了解ATP分布和复杂生物系统中的动态.

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