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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

24.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.6K
Alternative RNA Splicing02:18

Alternative RNA Splicing

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4.8K
RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.3K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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相关实验视频

Updated: Jan 11, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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量化EGAP元数据中的替代拼接中的注释驱动偏差.

Rebeca de la Fuente1, Wladimiro Díaz-Villanueva1,2,3, Vicente Arnau1,2,3

  • 1Foundation for the Promotion of Sanitary and Biomedical Research of the Valencian Community (FISABIO), 46020 Valencia, Spain.

NAR genomics and bioinformatics
|November 14, 2025
PubMed
概括

注释质量显著影响了真核生物中的替代拼接估计. 我们开发了一种规范化方法来纠正这种偏差,改进了比较基因组学分析.

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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科学领域:

  • 基因组学和生物信息学
  • 分子生物学分子生物学

背景情况:

  • 标注编码序列 (CDS) 对于估计全基因组的替代拼接至关重要.
  • 注释质量和证据支持可以在跨物种拼接事件估计中引入系统偏见.

研究的目的:

  • 评估NCBI真核生物基因组注释管道中的注释变量对推断的拼接水平的影响.
  • 开发一种方法来纠正对比基因组学的替代拼接估计中的注释驱动偏差.

主要方法:

  • 对670个多细胞真核生物基因组的分析.
  • 评价与注释相关的变量,包括具有实验证据的CDS的百分比,组装质量和原始的转录输入.
  • 使用多项式回归的规范化程序的开发.

主要成果:

  • 实验证据支持的CDS的百分比是拼接估计变化的主要预测因素.
  • 组装质量和原始转录基因输入在拼接估计变化中起到了很小的作用.
  • 为替代拼接生成了一个新的,调整的度量,减轻了注释文物.

结论:

  • 注释质量,特别是实验证据支持,是影响替代拼接估计的关键因素.
  • 开发的规范化程序为跨物种的比较拼接分析提供了更准确的指标.
  • 这项工作对提高比较基因组学研究的可靠性有重大影响.