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在神经板边界细胞命运规范期间,染色质可访问性的动态变化

Eva Hamrud¹, Jacob Leese¹, Alexandre P Thiery¹

¹Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral and Craniofacial Sciences, King's College London, London SE1 9RT, UK.

Development (Cambridge, England)

|November 14, 2025

概括

这项研究揭示了细胞在发育过程中如何做出命运决定,通过分析染色质可访问性的变化. 研究人员确定了特定的调控元素和转录因子,如Foxk2,控制细胞专业化.

科学领域:

发展生物学发展生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

细胞分化涉及动态基因表达和染色体重塑.
了解这些变化对于破译发育过程至关重要.

研究的目的:

为了研究小神经板边缘细胞发育期间的染色质可访问性变化.
识别细胞状态特定的调控元素和基因网络.

主要方法:

单细胞ATAC测序 (scATAC-seq) 和单细胞RNA测序 (scRNA-seq) 进行了整合.
为了数据分析,开发了一个Nextflow管道 ("单细胞高级染色体探索").
推断出以增强剂为中心的基因调控网络.

主要成果:

神经板边界细胞表现出广泛开放的染色质,表明多效.
在分化过程中,细胞类型特定的染色体特征出现.
Foxk2被确定为一个新型的调节器,其目标增强剂特别在斑点细胞中开放.

结论:

染色体可访问性动态在细胞命运决定中起着关键作用.
对于命运选择的监管策略包括差异增强器可访问性和转录因子驱动的广泛可访问性.

关键词:

一个漂亮的小.这一区域是Cis监管区域.福克斯K2 在线观看基因监管网络是基因监管网络.神经峰的神经峰神经板是神经板的一个组成部分.在Placodes中使用Placodes.

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