转录组测序揭示了Listeria monocytogenes 6179在对数和静止生长阶段的明显应激反应
Jessica L Strathman-Runyan1,2, Bienvenido W Tibbs-Cortes1,2,3, Stephan Schmitz-Esser1,2
1Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, Iowa, USA.
MicrobiologyOpen
|November 14, 2025
概括
李斯特菌单细胞菌的生长阶段显著影响其基因表达对食品生产环境压力的反应,如乳酸和氧化损伤. 与对数相细胞相比,静止相细胞显示出不同的反应.
科学领域:
- 食品安全微生物学 食品安全微生物学
- 细菌应激反应的细菌应激反应
- 基因表达分析 基因表达分析
背景情况:
- 李斯特菌 (Listeria monocytogenes) 是一种在食品生产环境 (FPE) 中发现的持久性食物传播病原体.
- 了解其承受压力的能力对于食品安全至关重要.
- 在L. monocytogenes中,生长阶段对应激反应基因表达的影响尚不清楚.
研究的目的:
- 在压力条件下研究生长阶段对Listeria monocytogenes基因表达的影响.
- 为了比较对乳酸和氧化应激的转录反应在对数和静止生长阶段之间的反应.
主要方法:
- 转录组测序 (RNA-Seq) 用于分析基因表达.
- 李斯特菌单细胞原体菌株6179暴露于乳酸和过氧化.
- 基因表达在对数和静止生长阶段进行了分析.
主要成果:
- 在增长阶段之间观察到不同的基因表达模式,以应对压力.
- 乳酸压力诱导了静止阶段的1809个差异表达基因,而在对数阶段的175个基因.
- 氧化应激诱导了静止阶段的184个差异表达基因,而在对数阶段的819个基因.
- 在两种压力下,对数相细胞都显示出共享的上调调节的基因,这些基因参与氨酸运输.
- 静止阶段细胞的基因表达更依赖于压力类型,许多上调的基因属于sB regulon.
结论:
- 生长阶段是调节Listeria monocytogenes对环境压力的反应的关键因素.
- 研究结果提供了有关病原体在食品生产环境中的适应机制的见解.
- 这些知识可以为控制L. monocytogenes持久性的策略提供信息.
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