在野生和化场生产的大珀 Seriola dumerili 呈现正常和受损的精子生成的不同垂体mRNA表达
Anna Lavecchia1, Caterina De Virgilio1, Caterina Manzari1
1Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Bari, Italy.
Animal reproduction science
|November 14, 2025
概括
在化场养大的大珀 (Seriola dumerili) 显示生殖问题. 在野生和被囚禁的雄性中,下垂体基因表达分析揭示了与精子生成受损相关的失调途径,这表明下垂体活动受到抑制.
科学领域:
- 水产养殖和生殖生物学
- 基因组学和分子生物学
- 鱼类生理学 鱼类生理学
背景情况:
- 大珀 (Seriola dumerili) 是地中海的一个关键水产养殖物种.
- 第一代 (F1) 化场生产的个体表现出生殖功能障碍.
- 囚禁会影响养殖鱼的生殖健康.
研究的目的:
- 调查F1大珀的生殖功能障碍背后的分子机制.
- 为了比较野外和化场养殖 (F1) 雄性大珀在精子生成过程中的垂体转录组.
- 确定关键的基因和受水产养殖环境中囚禁影响的途径.
主要方法:
- 下垂体转录组分析对野生 (WILD),正常F1 (NormalF) 和受损F1 (DysF) 的雄性大珀进行.
- 差异表达基因 (DEGs) 通过比较群体之间的转录组来确定.
- 进行了蛋白质与蛋白质相互作用 (PPI) 和基因和基因组京都百科全书 (KEGG) 途径分析.
主要成果:
- 转录组分析显示了野生和F1雄性之间基因表达的显著差异,在DysF与WILD之间有301个DEG,在NormalF与WILD之间有376个DEG.
- 在DysF和NormalF组之间没有发现DEG,这表明F1男性的共同点.
- 在F1雄性中,失调的通路与生殖功能,核酸代谢,信号传递,MAPK,mTOR和GnRH信号传递有关.
结论:
- 在F1大珀的生殖功能障碍可能源于脑下垂体活动的抑制.
- 对阿片类,内分泌类和多巴胺基系统的调节失调可能会调解垂体抑制.
- 研究结果提供了关于水产养殖物种生殖问题的分子基础的见解.
相关概念视频
01:11Testing a Claim about Mean: Known Population SD
3.2K
A complete procedure of testing the hypothesis about a population mean is explained here.
Estimating a population mean requires the samples to be distributed normally. The data should be collected from the randomly selected samples having no sampling bias. The sample size needed to be higher than 30, and most importantly, the population standard deviation should be already known.
In most realistic situations, the population standard deviation is often unknown, but in rare circumstances, when it...
Estimating a population mean requires the samples to be distributed normally. The data should be collected from the randomly selected samples having no sampling bias. The sample size needed to be higher than 30, and most importantly, the population standard deviation should be already known.
In most realistic situations, the population standard deviation is often unknown, but in rare circumstances, when it...
3.2K
02:27Background and Environment Affect Phenotype
7.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.4K