亡诱导和MAPK路径调节由Melanoma中的Lagerstroemia floribunda:实验和计算见解
Trang Thi Minh Nguyen1, Qiwen Zheng1, Xiangji Jin2
1Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, 17104, Republic of Korea.
Applied biochemistry and biotechnology
|November 14, 2025
概括
拉格斯特罗米亚花 (LF) 提取物显示出强大的抗氧化和抗黑色素瘤特性. 它通过破坏MAPK信号通路来抑制癌细胞的生长,迁移,并诱导细胞亡,从而提供了天然的治疗潜力.
科学领域:
- 植物化学和药理学
- 癌症生物学 癌症生物学
- 生物技术是生物技术.
背景情况:
- 黑色素瘤是一种具有高死亡率的侵袭性皮肤癌,推动了对新型治疗的需求.
- 拉格斯特罗米亚花 (Lagerstroemia floribunda,简称LF) 是一种植物,传统上被用于抗癌性质,这归因于其和黄含量.
- 天然产品为开发新的抗癌治疗策略提供了一个有前途的途径.
研究的目的:
- 为了研究Lagerstroemia floribunda (LF) 甲醇提取物的生化和抗黑色素瘤作用.
- 为了评估LF提取物的抗氧化能力.
- 阐明LF抗黑色素瘤活性背后的分子机制.
主要方法:
- 通过总/黄含量,SOD清理和ORAC测定来评估抗氧化活性.
- 评估了对B16-F10黑色素瘤细胞的抗增殖,殖民地形成和迁移抑制作用.
- 分析了MAPK信号通路调节 (JNK,ERK1/2,p38),细胞周期进展,亡诱导 (Bax,caspase-3/9) 和进行了分子对接研究.
主要成果:
- 黄提取物显示出显著的抗氧化活性.
- 黄提取物抑制了黑色素瘤细胞的增殖 (35.1%),殖民地形成 (30%) 和迁移 (21.32%).
- LF诱导了G2/M细胞周期停止,细胞亡 (98.5%),调节了MAPK信号传递,并上调了Bax/caspase通路. 奎尔因通过分子对接被确定为一种关键的生物活性化合物.
结论:
- 拉格斯特罗米亚花提取物具有显著的抗氧化和抗黑色素瘤特性.
- 乳腺癌有效地抑制黑色素瘤细胞的增殖,迁移,并通过调节MAPK信号和亡途径来诱导亡.
- 乳腺癌是开发新型黑色素瘤治疗药物的有希望的自然来源,支持基于天然产品的药物发现.
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