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Updated: Jul 14, 2026

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
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FlowLITE:一种使用流细胞计法来描述和量化人体血中总抗体同型的协议

Hyunjae J Hahm1, Alexandra F Nazzari2, Benjamin R Babcock1

  • 1Department of Medicine, Division of Immunology, Lowance Center for Human Immunology, Emory University, Atlanta, GA, USA.

STAR protocols
|November 14, 2025
PubMed
概括

本研究详细介绍了流细胞计光链和同型表达 (FlowLITE) 试验协议,用于量化人体血抗体光链和同型表达. 该方法允许使用小样本体积进行高通量分析.

关键词:
高通量选的高通量选抗体是一种抗体.免疫学 免疫学 免疫学

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相关概念视频

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...