RNA结合蛋白RBM20通过抑制Wt1 KTS的拼接来调节的温度依赖性性别决定
Wei Sun1,2, Hongzhe Yang3, Hangbo Hui1,2
1College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China.
Science advances
|November 14, 2025
概括
RNA结合动机蛋白20 (RBM20) 通过调节基因拼接来控制的性别决定. 在RBM20中使用RBM20
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 替代拼接与脊椎动物的性别决定有关,但机制尚不清楚.
- 爬行动物的温度依赖性性别决定 (TSD) 为研究环境对性发育的影响提供了一个模型.
- 特定RNA结合蛋白在TSD中的作用在很大程度上是未知的.
研究的目的:
- 调查RNA结合基因蛋白20 (RBM20) 在红耳滑 (Trachemys scripta) 温度依赖性性别确定中的作用.
- 阐明RBM20影响性别决定的分子机制.
- 为了确定替代拼接和脊椎动物的性别确定之间的因果关系.
主要方法:
- 在不同温度下检查了Trachemys scripta胚胎性腺中的RBM20表达模式.
- 在雄性和雌性生产温度下利用了Rbm20的敲除和过度表达技术.
- 分析了RBM20对威尔姆斯瘤抑制剂WT1及其异型的剪接的影响.
主要成果:
- 在早期的生殖腺中,RBM20表现出温度依赖性,两形性表达.
- 在男性生殖温度下降导致男性对女性的性别逆转.
- 在雌性生产温度下过度表达诱导了雄性发育,证明了RBM20的关键作用.
- 发现RBM20抑制了KTS位点上的WT1的拼接,控制了+KTS/-KTS异形平衡.
- 确定了Wt1+KTS变种是必要的和足够的,用于该物种的雄性发育.
结论:
- 通过调节WT1拼接,RBM20作为Trachemys scripta性别确定中的关键调节剂.
- RBM20通过抑制Wt1 KTS拼接激活男性通路,建立直接的遗传联系.
- 这项研究提供了第一个因果证据,证明了替代拼接在脊椎动物性别决定中的作用.
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