转录因子MYB78负面调节了Actinidia chinensis的寒冷应激耐受性
Wangmei Ren1, Lei Cao2, Linyao Liu1
1School of Horticulture, Anhui Agricultural University, Hefei 230036, China.
Plant physiology
|November 14, 2025
概括
果AcMYB78通过抑制C重复结合因子 (CBF) 和激活AcRAP2.1.1,从而负面调节寒冷耐受性. 这是一架MYB78飞机.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- C-重复结合因子 (CBF) 路径对于冷应激耐受性至关重要.
- 在果 (Actinidia chinensis) 中,AcCBF及其调节者的作用在很大程度上没有被描述.
研究的目的:
- 调查AcMYB78在果寒冷应激反应中的功能和调节.
- 阐明AcMYB78影响寒冷耐受性的分子机制.
主要方法:
- 基因表达分析 (qPCR)
- 酵母单杂交 (Y1H) 的测定方法
- 双 luciferase 记者测定 测定 测定 测定
- 染色体-免疫沉-qPCR (ChIP-qPCR) 进行检测.
- 电泳运动移动性转换试验 (EMSA)
- 阿拉比多普西斯塔利亚纳功能分析.
主要成果:
- 在果中,AcMYB78作为寒冷和结耐受性的负调节剂.
- 在激活AcRAP2.1.1,AcMYB78直接抑制AcCBF1和AcCBF2的表达,同时激活AcRAP2.1.
- 过度表达AcMYB78降低了寒冷耐受性,而突变者则表现出增强的耐受性.
- 在冷应激反应期间,MYB78的表达模式会动态变化.
- 在Arabidopsis thaliana中,AtMYB78表现出类似的负调节功能.
结论:
- AcMYB78是基维果寒冷压力耐受性的关键负调节剂.
- AcMYB78通过调节CBF和RAP2.1的表达来起作用,影响下游冷调节 (COR) 基因.
- 了解MYB78的作用,可以了解植物的寒冷适应机制.
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