MoRNiNG:与RNA二级结构动力学相关的RNA修饰站点的数据库
Yicen Zhou1, Shanxin Lyu1, Shiau Wei Liew2
1Department of Biomedical Sciences, College of Biomedicine, City University of Hong Kong, Hong Kong Special Administrative Region 999077, China.
Genomics, proteomics & bioinformatics
|November 14, 2025
概括
像N6-甲基氨酸 (m6A) 这样的RNA修改可以切换RNA结构,影响基因调节. 一个新的数据库,MoRNiNG,在G-四重复结构中对这些RNA修饰进行了目录.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 在RNA生物学,RNA生物学.
背景情况:
- RNA分子表现出超出正规干循环的动态次要结构,包括G-四复合体 (rG4s).
- 了解序列相同的RNA如何采用不同的结构,以及这些转换是如何调节的,对于破译转录组功能至关重要.
- 越来越多的RNA修饰被认为是RNA结构和功能的关键调节者.
研究的目的:
- 研究RNA修饰对RNA二次结构,特别是rG4s的形成和转变的影响.
- 在转录组中识别具有多面结构形成潜力的RNA段.
- 在自然rG4序列中开发RNA修饰的综合数据库.
主要方法:
- 生物信息分析以识别假设的G-四重复形成序列 (PQS) 和相关的RNA修饰部位.
- 开发MoRNiNG数据库,包括修改站点解决方案的可靠性级别.
- 实验验证了N6-甲基亚氨酸 (m6A),5-甲基亚氨酸 (m5C) 和氨酸对氨酸 (A-to-I) 编辑对rG4形成的影响.
主要成果:
- 跨越转录组的许多RNA段具有形成多个不同的结构的潜力.
- 证明N6-甲基氨酸 (m6A) 修改影响HOXB9RNA结构和RNA结合蛋白 (RBP) 相互作用.
- 实验验证证证实m6A,m5C和A-to-I编辑可以作为调节rG4形成的"修改开关".
结论:
- 来自同一序列的RNA结构的多样性和动态转换在RNA调节中起着重要作用.
- RNA修饰是RNA结构的关键调节者,影响G-四重复形成和潜在的其他结构构造.
- MoRNiNG数据库为研究自然rG4s中的RNA修饰提供了宝贵的资源,并促进了我们对RNA结构动态的理解.
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