Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

In Vitro Fertilization01:24

In Vitro Fertilization

868
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
868

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Resilience of lung grafts to warm ischemia: assessment by <i>ex vivo</i> perfusion using functional and molecular analysis.

Transplant international : official journal of the European Society for Organ Transplantation·2026
Same author

Placental adaptation to maternal environment in domestic animals.

The Journal of reproduction and development·2026
Same author

Tissue clearing and light-sheet fluorescence microscopy for three-dimensional mapping of placental vascularization.

Placenta·2026
Same author

Erratum: N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation.

Molecular therapy. Nucleic acids·2026
Same author

N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation.

Molecular therapy. Nucleic acids·2026
Same author

Contrasted modifications of IgM and IgT repertoires induced by high- and low-virulent infectious pancreatic necrosis virus strains in rainbow trout (<i>Oncorhynchus mykiss</i>).

Frontiers in immunology·2026

相关实验视频

Updated: Jan 11, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

8.1K

转录基因分析显示,马类IVF和ICSI胚胎之间存在相似之处.

Klaartje Broothaers1, Alice Jouneau2, Daniel Angel-Velez3

  • 1Department of Internal Medicine, Reproduction and Population Medicine, ReproGenT, Ghent University, Merelbeke, Belgium.

Theriogenology
|November 14, 2025
PubMed
概括

马类体外受精 (IVF) 和细胞内精子注射 (ICSI) 在胚胎中表现出类似的基因表达. ICSI导致更高的胚胎细胞发育率,这表明试管婴儿对于马类生殖研究是安全的.

关键词:
胚胎 胚胎是一个胚胎.一个闪光灯-seqq.在受精过程中进行受精.马马的马,就是马马.在IVP中,IVP是IVP.转录组 转录组就是一个转录组.

更多相关视频

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
12:11

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

11.4K
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
12:32

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

2.6K

相关实验视频

Last Updated: Jan 11, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

8.1K
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
12:11

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

11.4K
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
12:32

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

2.6K

科学领域:

  • 兽医繁殖 兽医繁殖 兽医繁殖
  • 胚胎学 胚胎学
  • 基因组学就是基因组学.

背景情况:

  • 20年来,细胞体内精子注射 (ICSI) 是马类胚胎生产的主要方法.
  • 传统的体外受精 (IVF) 是一种新的,成功的马胚胎生产替代方案.
  • 受精方法对马类胚胎质量和基因表达的影响在很大程度上仍未得到研究.

研究的目的:

  • 为了比较通过ICSI生产的马类胚胎的转录组概况与IVF.
  • 在ICSI和试管婴儿胚胎之间识别差异表达基因 (DEG).
  • 评估受精方法对马类胚胎发育和质量的影响.

主要方法:

  • 通过ICSI或试管婴儿从三只马匹中产生了十个兄弟的马类胚胎囊.
  • 进行了全长单细胞RNA测序 (FLASH测序) 在胚芽细胞上.
  • 分析了裂变和胚胎细胞发育率,以及胚胎细胞发育的日期.

主要成果:

  • 在ICSI和试管婴儿胚胎之间没有发现差异表达基因 (DEG),总共确定了11,518个基因.
  • 在试管婴儿 (55.0%) 和ICSI (51.9%) 中,切割率相似.
  • 在ICSI (71.4%) 后,胚胎细胞的发病率明显高于IVF (40.9%) 在裂开的胚胎细胞中 (P=0.004).

结论:

  • 马类试管婴儿和ICSI导致由此产生的胚胎具有类似的基因表达特征.
  • 虽然ICSI的胚胎细胞发育率更高,但类似的基因表达支持马类试管婴儿临床应用的安全性.
  • 马作为研究辅助生殖技术的相关模型,对人类医学有潜在的影响.