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由Trichoderma viride产生的银纳米颗粒的细胞内和细胞外生物合成,表征和抗菌和抗生素去除活动

Qiaoqing Xie¹, Mingyang Song¹, Xiaoping Lin¹

¹State Key Laboratory forPollution Control and Resource Reuse, College of Environmental Science andEngineering, Tongji University, Shanghai 200092, China.

Colloids and surfaces. B, Biointerfaces

|November 14, 2025

概括

这项研究揭示了营养变化如何影响银纳米颗粒 (AgNPs) 的真菌生物合成. 不同的条件产生细胞内或细胞外AgNP,具有不同的抗微生物和污染物去除能力.

科学领域:

纳米材料科学纳米材料科学
微生物学微生物学
环境科学环境科学

背景情况:

生物合成为各种应用提供了纳米材料的可持续生产.
微生物的营养状况极大地影响了生物合成过程.
银纳米粒子 (AgNPs) 在生物医学和环境修复方面表现有前途.

研究的目的:

研究营养环境如何调节Trichoderma viride (T. viride) 中的细胞内和细胞外AgNP生物合成.
阐明受营养因素影响的AgNP生物合成的潜在机制.
评估生物合成的AgNP的抗菌疗效和环境应用潜力.

主要方法:

使用倾斜扫描传输电子显微镜 (T-STEM) 和冷传输电子显微镜 (Cryo-TEM) 来阐明机制.
采用蛋白质表达分析来识别参与AgNP合成的关键生物分子.
对精选的细菌物种进行了抗菌检测 (大肠杆菌,B. subtilis,Shewanella sp. ) 的情况.

主要成果:

有机丰富的介质有利于通过细胞表面吸附和减少进行细胞外AgNP合成,与高调节的还原蛋白相关.
无机盐基介质促进了细胞内AgNP合成和细胞外形成,与囊泡运输和戈尔吉器官蛋白质相关.
生物合成的AgNP表现出显著的抗菌活性,并实现了近100%的抗生素污染物从水中去除.

结论:

营养条件对生物合成的AgNP的位置 (细胞内与细胞外) 和特征产生深远的影响.
了解这些机制可以提高针对特定应用的AgNP的有针对性的生产.
生物合成的AgNP具有显著的环境修复潜力,特别是在清除水污染物方面.

关键词:

低温传输电子显微镜的使用.细胞外/细胞内合成蛋白质表达分析分析蛋白质表达分析银纳米颗粒银纳米粒子这种虫是Trichoderma viridie.

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