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一种基于MALDI生物型的快速方法,用于查孕妇中Streptococcus agalactiae的阴道外殖民

Suellen Bernardo de Queiroz¹, Carlos Gabriel Andrade Barbosa¹, Marcelo Henrique da Silva Pereira¹

¹Núcleo de Medicina Tropical - NUMETROP, Centro de Ciências da Saúde, Universidade Federal da Paraíba, Cidade Universitária Street, João Pessoa, PB, Brazil.

Journal of microbiological methods

|November 14, 2025

概括

一个新的快速测试,MALDI-Biotyper快速B组识别试验 (MBT-RBA),准确地检测孕妇的Streptococcus agalactiae殖民. 这种方法提供了一个更快的查选择,以防止新生儿疾病的传播.

科学领域:

微生物学微生物学
临床诊断临床诊断临床诊断
公共卫生公共卫生

背景情况:

母亲对Streptococcus agalactiae (B组链球菌) 的殖民是向新生儿垂直传播的主要危险因素.
早期发病的新生儿疾病可能是垂直传播的结果,这凸显了有效查的必要性.
目前的查方法指导产后抗生素的使用,以减轻疾病的风险.

研究的目的:

引入和评估一种新的协议,即MALDI-Biotyper快速B组鉴定试验 (MBT-RBA),用于检测母体S. agalactiae殖民.
用MALDI-TOF平台直接从缩中评估MBT-RBA的性能.

主要方法:

在混合培养中测试了MBT-RBA协议检测S. agalactiae的能力.
性能与标准培养方法进行了比较,使用来自孕妇的137个无阴道拭子.
通过使用cfb基因的qPCR放大证实了MBT-RBA的积极结果.

主要成果:

在混合培养中,MBT-RBA在检测S. agalactiae方面表现出高度的信心和准确性.
发现母体殖民的流行率为17.5%.
MBT-RBA 实现了75%的灵敏度,100%的特异性,100%的PPV和94.9%的NPV.

结论:

该MBT-RBA协议可在化6小时内可靠地检测阴道外殖民.
这种快速检测是一种可行的和有价值的工具,用于查孕妇.
MBT-RBA 作为对现有文化或分子方法进行补充测试,用于评估殖民状态.

关键词:

早期发病的疾病早期发病.B组的链球菌.怀孕怀孕怀孕怀孕在产前,产前生育.

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