对三种多重免疫试验进行比较,用于在角质层 (stratum corneum) 的磁带上分析生物标志物
Nariman K A Metwally1,2,3, Florentine de Boer1, Jop Vreeken4
1Public and Occupational Health Department, Amsterdam UMC, University of Amsterdam, Amsterdam Public Health Research, Amsterdam, The Netherlands.
Scientific reports
|November 14, 2025
概括
梅索尺度发现 (MSD),NULISA和Olink平台在皮肤带中显示出不同的蛋白质检测灵敏度. MSD为皮肤炎研究提供了最高的灵敏度和绝对量化.
科学领域:
- 生物化学 生化学
- 皮肤病学 皮肤病学
- 分析化学 分析化学
背景情况:
- 皮层角膜带提供非侵入性皮肤采样,但面临着低蛋白质产量的挑战.
- 多重免疫测试平台对于同时检测多个蛋白质生物标志物至关重要.
研究的目的:
- 为了比较三个多重免疫测试平台的性能:Meso Scale Discovery (MSD),NULISA和Olink.
- 为了评估它们在检测角层皮肤中的蛋白质标记物的灵敏度和一致性,从健康的皮肤和受皮肤炎影响的皮肤上取出带.
主要方法:
- 在MSD,NULISA和Olink平台上评估了30种共享蛋白质,使用层状角膜带样本.
- 根据超过蛋白质特定检测极限的样本>50%的门,评估蛋白质检测能力.
- 分析了非损伤性皮肤,刺激性接触性皮肤炎,过敏性接触性皮肤炎和临床手部皮肤炎的样本.
主要成果:
- 骨髓硬化表现出最高的敏感性 (检测到70%的共享蛋白质),其次是NULISA (30%) 和Olink (16.7%).
- 四种蛋白质 (CXCL8,VEGFA,IL18,CCL2) 在所有平台上都得到了一致的检测,具有良好的类间相关性 (0.50.86).
- 所有平台在对照和皮肤炎受影响的皮肤之间显示了类似的差异性蛋白质表达模式,表明了整体一致性.
结论:
- MSD在角质层的带中表现出对蛋白质检测的优越灵敏度.
- 虽然所有平台在微分表达中都显示出一致性,但MSD独特地提供了对正常化的绝对量化.
- NULISA和Olink适用于较小的样本体积和较少的测试运行,但需要进一步研究以获得更广泛的适用性.
相关概念视频
01:20Immunogold Electron Microscopy
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
01:13Multiple Comparison Tests
Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...