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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

对MINFLUX显微镜的参数优化启用了单颗粒追踪.

Bela T L Vogler^1,2,3, Giovanni De Angelis^4,5, Ziliang Zhao^4,5,6

¹Institute of Applied Optics and Biophysics, Friedrich-Schiller University Jena, Jena, Germany. bela.vogler@uni-jena.de.

Communications biology

|November 14, 2025

概括

MINFLUX 超高分辨率显微镜可实现细胞动态的实时单颗粒追踪 (SPT). 这项研究介绍了MINFLUX的参数优化策略,成功追踪了膜中的快速脂质扩散.

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Last Updated: Jan 11, 2026

A Protocol for Real-time 3D Single Particle Tracking

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Published on: January 3, 2018

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科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
显微镜的使用方法

背景情况:

MINFLUX (MFX) 是一种尖端的超高分辨率显微镜技术.
它允许详细研究细胞结构和动态.
MINFLUX 可实现实时单颗粒追踪 (SPT).

研究的目的:

为MINFLUX提出一个参数优化策略.
提供MINFLUX关键参数的概述.
为了证明MFX-enabled SPT的快速扩散的膜脂质.

主要方法:

为MINFLUX开发一个参数优化策略.
可追踪扩散速率的理论分析.
MINFLUX SPT对膜脂质的实验应用.

主要成果:

介绍了一个优化MINFLUX参数的策略.
确定了可追踪扩散速率的理论极限.
MINFLUX成功地追踪了膜中的脂质的快速横向布朗运动 (D_MSD = 2.5 μm2/s).

结论:

优化的MINFLUX参数对于准确的测量至关重要.
MINFLUX能够追踪细胞膜中的快速分子动态.
这项工作推动了超分辨率显微镜在细胞生物学中的应用.