高温耐受异型化细菌Aeribacillus pallidus sp.有效利用的途径和机制 GW-E GW-E 的意思是什么?
Yindi Zhang1, Yongqi Ma1, Wenrui Qi1
1College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
BMC microbiology
|November 14, 2025
概括
一种新型的热友细菌,Aeribacillus pallidus sp. 这种新型的热友细菌. GW-E,在高温下有效地转化化合物. 在优化条件下,实现了100%的-利用率,突出显示了它在堆肥应用中的潜力.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 高温堆肥系统需要有效的管理,以防止营养损失.
- 热友细菌在高温下营养循环中起着至关重要的作用.
研究的目的:
- 从有氧堆肥中分离和描述一种新型热友异质化细菌.
- 为了研究隔离菌株的代谢和最佳生长条件.
- 为了评估细菌在高温堆肥中保持的潜力.
主要方法:
- 一种新型细菌菌株的隔离和鉴定 (Aeribacillus pallidus sp. 在GW-E).
- 全基因组测序以确定代谢和热应激基因.
- 响应表面的方法来优化环境条件 (C/N比,温度,pH).
- 酶活性测定和平衡分析.
主要成果:
- 艾里巴西勒斯 (Aeribacillus pallidus) sp. 这种细菌的种类. 在55°C时,GW-E证明了NH4+-N (87.42%),NO3−-N (21.44%) 和NO2−-N (51.68%) 的有效利用.
- 在最佳条件下 (C/N比为15,54°C,pH值为8),NH4+-N的利用率达到100%.
- 确定了关键的代谢和热应激基因.
- 氨同化通路酶 (GS,GDH,GOGAT) 的活性显著提高.
结论:
- 艾里巴西勒斯 (Aeribacillus pallidus) sp. 这种细菌的种类. GW-E主要使用氨同化进行转化,并补充了酸盐减少和化.
- 这种细菌是高温堆肥中保留的宝贵微生物资源.
- 这项研究为增强堆肥系统中管理提供了理论基础.
更多相关视频
10:23Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
Published on: August 23, 2024
1.6K
07:26Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
25.0K
相关概念视频
Inorganic Nitrogen Assimilation
441
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
441
Carbon-dioxide Fixation
610
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
610
Overview of Nitrogen Metabolism
10.9K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
10.9K
Amino Acid Catabolism
960
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
960
Other Glycolytic Pathways
794
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
794
Metabolism of Chemolithotrophs
741
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
741
