从牛中分离出来的沙门氏菌菌株中的抗生素敏感性模式和耐药性基因
Muhammad Zeeshan Malik1, Saeed Ul Hassan Khan2, Hamid Irshad3
1Department of Zoology, Quaid I Azam University, Islamabad, Pakistan.
BMC veterinary research
|November 14, 2025
概括
巴基斯坦牛的沙门氏菌对抗生素的耐药性是一个主要问题. 观察到对氧基环和多环的高耐药性,表明需要替代治疗和持续监测.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 食品安全 食品安全
背景情况:
- 来自巴基斯坦牛的沙门氏菌株的抗生素耐药性是一个重大的公共卫生问题.
- 牛群中的沙门氏菌感染可能会影响动物健康,并可能传播给人类.
- 了解耐药性模式对于有效的治疗和控制策略至关重要.
研究的目的:
- 为了确定来自巴基斯坦牛的沙门氏菌分离物的抗生素敏感性模式.
- 为了确定这些隔离物中特定抗生素耐药性基因的流行率.
- 为畜牧业提供数据,以提供知情的抗微生物管理.
主要方法:
- 从巴基斯坦的奶牛农场收集了100头牛便样本.
- 使用生物化学测试和InvA基因PCR确认了沙门氏菌分离物.
- 通过盘扩散评估抗生素敏感性,并选耐药基因 (tetA,tetC,blaTEM-1等). 使用PCR的方法.
主要成果:
- 28%的样本产生了沙门氏菌分离物.
- 100%的抗氧四环素和多西环素;64%的抗安培素.
- 普遍存在的耐药基因包括tetA (25%),blaTEM-1 (18%) 和tetC (3.6%).
- 在一些没有可检测的基因的隔离物中观察到表型耐药性.
结论:
- 对氧基环和多环的高耐药性需要替代治疗方法.
- 该研究强调需要继续监测牛的抗菌素耐药性.
- 这些发现强调了有针对性的控制措施的重要性,以减轻耐药沙门氏菌株的传播.
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