一个分析策略可靠的代谢分析的临床剩余血清使用定时的引用
Deying Chen1, Shuang Zhao2, Guanghua Ma1
1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Analytical chemistry
|November 15, 2025
概括
临床剩余血清可可靠地用于非目标代谢. 定时的引用缩短了分析前的变化,使得从存储的样本中能够稳定地发现生物标志物.
科学领域:
- 代谢学 代谢学 代谢学
- 临床化学 临床化学
- 生物标志物发现发现
背景情况:
- 准确的代谢分析需要严格控制预分析变量.
- 临床剩余血清是一种有价值的资源,但由于储存时间和量化时间的变化,容易受到代谢物降解.
研究的目的:
- 开发和验证使用临床剩余血清可靠的非目标代谢学分析策略.
- 评估定时位和短期存储对代谢物稳定性和代谢资料的影响.
主要方法:
- 使用高覆盖率的12C-/13C-dansylationLC-MS进行亚代谢形状.
- 在多个时间点对健康个体的血清样本进行分析后抽取 (发现和验证集).
- 在众多LC-MS运行中量化了大量代谢物 (1382和1352).
主要成果:
- 在相邻的时间点之间观察到相对较小的代谢物丰度的时间依赖变化.
- 在24小时间隔内标记样本时,表现出明显的基于性别的代谢分离.
- 在组合具有较长存储时间差异的样本时,发现歧视力下降.
结论:
- 临床剩余血清可以可靠地用于代谢学,并经过精心定时的标配.
- 尽量减少存储时间差异对于控制分析前变异至关重要.
- 建立了一个实用的工作流程,以便在人口规模的代谢学和生物标志物发现中更广泛地使用临床样本.
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