血中的乌拉:两种内部开发的LC-MS/MS方法的比较
Svend H Hansen1, Eva Greibe2,3
1Department of Clinical Biochemistry, 53146 Copenhagen University Hospital, Rigshospitalet , Copenhagen, Denmark.
Clinical chemistry and laboratory medicine
|November 15, 2025
概括
两种新的染色学方法准确地测量了用于5-甲 (5-FU) 化疗监测的血 uracil 水平. 这些方法显示出强烈的相关性和一致的临床解释,有助于预防5-FU中毒.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 药理动力学 药理动力学
背景情况:
- 欧洲药物管理局建议进行血 uracil 测量,以防止在化疗期间发生 5-fluorouracil (5-FU) 中毒.
- 准确可靠的分析方法对于对5-FU的治疗药物监测至关重要.
研究的目的:
- 介绍和比较两种用于血 uracil 分析的新型染色学方法.
- 证明开发的临床解释方法的协调性和可比性.
主要方法:
- 两个独立的实验室开发并验证了具有质谱检测的内部染色学方法.
- 方法1在蛋白质沉后使用水友互动液体色谱 (HILIC).
- 方法2在蛋白质沉,过,蒸发和制后使用C18逆相色谱.
主要成果:
- 这两种方法都在血样本中提供了稳健而准确的 uracil 量化.
- 在使用161名患者样本的两种方法之间观察到强烈的相关性 (2%的相对平均偏差).
- 大多数样本 (150/161) 的临床解释一致,其中11个样本接近临床决定极限.
结论:
- 提出的简化染色学方法准确量化血 uracil,而不会显著影响临床解释.
- 确定了14-20μg/L的潜在分析不准确范围,包括临床决定的下限 (16μg/L).
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