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Updated: Jan 11, 2026

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Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
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颗粒物诱导的NETosis通过中性粒细胞的pexophagy促进过氧体降解
Yeji Shin1, Kahyun Kim1, Jisoo Han2
1Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea.
Ecotoxicology and environmental safety
|November 15, 2025
概括
暴露于颗粒物 (PM) 会触发中性粒细胞外细胞陷 (NETs),并破坏过氧体内平衡. 这项研究揭示了一个ROS-NET-过氧体轴驱动PM诱导的免疫失调.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 环境健康 环境健康
背景情况:
- 中性粒细胞外细胞陷 (NETs) 对于宿主防御至关重要,但在失调时可能导致炎症.
- 空气污染物颗粒物 (PM) 诱导了NET的形成,但其对中性粒细胞 peroxisomal homeostasis的影响尚不清楚.
研究的目的:
- 为了研究 PM 暴露对中性粒细胞内 NETosis 和 peroxisomal 动态的影响.
- 阐明PM,NET和过氧体功能障碍之间的分子机制.
主要方法:
- 骨髓衍生中性粒细胞 (BMDNs) 和分化的HL-60细胞暴露于PM.
- 对PM处理的BMDN进行了RNA测序.
- 分析了反应性氧物种 (ROS) 水平,NETosis标记物 (PAD4,citH3) 和过氧体标记物.
- 评估了食性标记物和LC3B与PEX13的同地化.
主要成果:
- 暴露于颗粒物上调了NETosis基因 (Padi4,Mpo) 和过氧体降解基因 (Pex5,Nbr1,Map1lc3b).
- PM增加了ROS,诱导了依赖ROS的NETosis和动态调节的过氧体标志物.
- 食被激活,与ROS依赖的NET形成相关,并被ROS/NET抑制剂减弱.
结论:
- 暴露于颗粒物会诱导中性粒细胞中的ROS-NET-氧体轴.
- 这个轴在环境压力下有助于免疫失调.
- 针对这种途径可能为PM诱导的炎症提供治疗策略.
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